Aguilar Lucero Dianela, Cantoia Alejo, Ceccarelli Eduardo A, Rosano Germán L
Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Instituto de Biología Molecular y Celular de Rosario (IBR), CONICET, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina.
Methods Enzymol. 2021;659:3-18. doi: 10.1016/bs.mie.2021.08.019. Epub 2021 Sep 22.
One of the goals in recombinant protein production in Escherichia coli is to maximize productivity. High volumetric and specific yields can be reached after careful selection of expression strains and optimization of cultivation parameters. In this chapter, we review the many tools available to make the most out of this versatile microbial cell factory. Useful guidelines and options for troubleshooting production are presented.
在大肠杆菌中生产重组蛋白的目标之一是实现生产力最大化。在仔细选择表达菌株并优化培养参数后，可以实现高体积产量和比产量。在本章中，我们将回顾许多可用于充分利用这个多功能微生物细胞工厂的工具。还将介绍生产故障排除的有用指南和选项。
