Key Laboratory of New Materials and Facilities for Rural Renewable Energy of Ministry of Agriculture and Rural Affairs of China, Henan Agricultural University, Zhengzhou 450002, PR China.
Key Laboratory of New Materials and Facilities for Rural Renewable Energy of Ministry of Agriculture and Rural Affairs of China, Henan Agricultural University, Zhengzhou 450002, PR China; Institute of Agricultural engineering, Huanghe S & T University, Zhengzhou 450006, PR China.
Bioresour Technol. 2022 Jan;344(Pt B):126302. doi: 10.1016/j.biortech.2021.126302. Epub 2021 Nov 6.
Suitable illumination project would help in achieving high light conversion efficiency (LCE) for photo-fermentation. This study proposed an improvement strategy for LCE of photo-fermentative hydrogen production (PFHP) with a photosynthetic consortium by adopting light-dark duration alternation. For this purpose, 6 projects (continues light, 24 h light + 24 h dark, 24 h dark + 24 h light, 48 h light + 48 h light, 48 h dark + 48 h light, and continues dark) light disturbances were carried out to estimate the strategy. The fluctuation of cell growth (OD660) was corresponded to the light-dark alternation. 24 h dark + 24 h light alternation achieved the maximum hydrogen yield (HY) of 390.9 mL/g TS cell (6.7 % higher than continuous light) and maximum improvement of LCE of 114.7%. Moreover, heat map analysis revealed that the light period after inoculation had the closest relation (Pearson's r = 1) with the average hydrogen production rate (HPR) of photo-fermentation. Besides, decreased dark period after inoculation would increase the hydrogen yield of photo-fermentation.
合适的光照方案有助于实现光合发酵的高光转化效率（LCE）。本研究通过采用光暗时长交替提出了一种提高光合产氢（PFHP）LCE 的改进策略，该策略采用光合生物群落。为此，进行了 6 个项目（连续光照、24 h 光照+24 h 黑暗、24 h 黑暗+24 h 光照、48 h 光照+48 h 光照、48 h 黑暗+48 h 光照和连续黑暗）的光照干扰，以评估该策略。细胞生长（OD660）的波动与光暗交替相对应。24 h 黑暗+24 h 光照交替实现了最大的氢气产量（HY）390.9 mL/g TS 细胞（比连续光照高 6.7%）和 LCE 的最大提高 114.7%。此外，热图分析显示，接种后光期与平均产氢率（HPR）之间存在最密切的关系（Pearson r=1）。此外，接种后黑暗期的缩短会增加光合发酵的氢气产量。
