Henan Agricultural University, Henan International Joint Laboratory of Biomass Energy and Nanomaterials, Zhengzhou 450002, China.
Key Laboratory of Environmental and Applied Microbiology, Environmental Microbiology Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.
Bioresour Technol. 2023 Jun;377:128931. doi: 10.1016/j.biortech.2023.128931. Epub 2023 Mar 20.
Bacterial immobilization is a common method in anaerobic fermentation, since of the maintenance of high bacterial activity, insurance of high density microbial during continuous fermentation, and quick adaptability to the environment. While, the bio-hydrogen production capacity of immobilized photosynthetic bacteria (I-PSB) is seriously affected by the low light transfer efficiency. Hence, in this study, photo-catalytic nano-particles (PNPs) was added into the photo-fermentative bio-hydrogen production (PFHP) system, and its enhancement effects of bio-hydrogen production performance were investigated. Results showed that the maximum cumulative hydrogen yield (CHY) of I-PSB with 100 mg/L nano-SnO (154.33 ± 7.33 mL) addition was 18.54% and 33.06% higher than those of I-PSB without nano-SnO addition and control group (free cells), and the lag time was the shortest indicating a shorter cell arrest time, more cells and faster response. Maximum energy recovery efficiency and light conversion efficiency were also found to be increased by 18.5% and 12.4%, respectively.
细菌固定化是厌氧发酵中常用的方法，因为它可以维持高细菌活性、在连续发酵过程中保证高密度微生物、并快速适应环境。然而，固定化光合细菌（I-PSB）的生物产氢能力受到低光传递效率的严重影响。因此，在本研究中，将光催化纳米颗粒（PNPs）添加到光发酵生物制氢（PFHP）系统中，研究了其对生物制氢性能的增强效果。结果表明，添加 100mg/L 纳米 SnO 的 I-PSB 的最大累积产氢量（CHY）为 154.33±7.33mL，分别比未添加纳米 SnO 的 I-PSB 和对照组（游离细胞）高 18.54%和 33.06%，且迟滞时间最短，表明细胞停滞时间更短、细胞更多、响应更快。还发现最大能量回收效率和光转换效率分别提高了 18.5%和 12.4%。
