Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Changsari, 781101, India; National Centre for Pharmacoengineering, NIPER, Guwahati, Changsari, 781101, India.
Department of Pharmaceutical Analysis, National Institute of Pharmaceutical Education and Research (NIPER), Guwahati, Changsari, 781101, India.
Anal Chim Acta. 2021 Dec 1;1187:339142. doi: 10.1016/j.aca.2021.339142. Epub 2021 Oct 8.
Analytical sample preparation techniques are regarded as crucial steps for analyzing compounds from different biological matrices. The development of new extraction techniques is a modern trend in the bioanalytical sciences. 3D printed techniques have emerged as a valuable technology for prototyping devices in customized shapes for a cost-effective way to advance analytical sample preparation techniques. The present study aims to fabricate customized filaments through the hot-melt extrusion (HME) technique followed by fused deposition modeling mediated 3D printing process for rapid prototyping of 3D printed sorbents to extract a sample from human plasma. Thus, we fabricated our own indigenous filament using poly (vinyl alcohol), Eudragit® RSPO, and tri-ethyl citrate through HME to prototype the fabricated filament into a 3D printed sorbent for the extraction of small molecules. The 3D sorbent was applied to extract hydrocortisone from human plasma and analyzed using a validated LC-MS/MS method. The extraction procedure was optimized, and the parameters influencing the sorbent extraction were systematically investigated. The extraction recovery of hydrocortisone was found to be >82% at low, medium, and high quality control samples, with a relative standard deviation of <2%. The intra-and inter-day precisions for hydrocortisone ranged from 1.0% to 12% and 2.0%-10.0%, respectively, whereas the intra-and inter-day accuracy for hydrocortisone ranged from 93.0% to 111.0% and 92.0% to 110.0%, respectively. The newly customizable size and shape of the 3D printed sorbent opens new possibilities for extracting small molecules from human plasma.
分析样品制备技术被认为是分析来自不同生物基质的化合物的关键步骤。开发新的提取技术是生物分析科学的现代趋势。3D 打印技术已成为一种有价值的技术，可以通过成本效益高的方式制造定制形状的原型设备，从而推进分析样品制备技术的发展。本研究旨在通过热熔挤出（HME）技术制造定制的纤维，然后通过熔融沉积建模介导的 3D 打印工艺对其进行快速原型制作，以制造用于从人血浆中提取样品的 3D 打印吸附剂。因此，我们使用聚（乙醇）、Eudragit®RSPO 和三乙基柠檬酸通过 HME 制造了自己的本土纤维，然后将其原型纤维制成用于小分子提取的 3D 打印吸附剂。将 3D 吸附剂应用于从人血浆中提取氢化可的松，并使用经过验证的 LC-MS/MS 方法进行分析。优化了提取程序，并系统地研究了影响吸附剂提取的参数。在低、中、高质量控制样品中，氢化可的松的提取回收率均>82%，相对标准偏差<2%。氢化可的松的日内和日间精密度分别为 1.0%-12%和 2.0%-10.0%，而氢化可的松的日内和日间准确度分别为 93.0%-111.0%和 92.0%-110.0%。3D 打印吸附剂新的可定制尺寸和形状为从小分子从人血浆中提取提供了新的可能性。
