Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun, P.R. China.
College of Food Science and Engineering, Jilin Agricultural University, Changchun, P.R. China.
J Food Biochem. 2021 Dec;45(12):4892-4902. doi: 10.1111/jfbc.13943. Epub 2021 Nov 10.
Malvids anthocyanins have been proven to have a significant antioxidant activity. However, natural anthocyanins are unstable as they are easily affected by temperature, light, and pH. They can produce copigmentation with caffeic acids, leading to the improvement of color stability. The objective of this research was to survey the anti-oxidative stress functional role of stabilization malvids anthocyanins (SMA) in vivo. Changes on the superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), total antioxidant capacity (T-AOC) and malondialdehyde (MDA) in the serum and liver of oxidatively damaged mice of SMA were investigated. The effects of SMA on the diversity of gut microbiota in mice with oxidatively damage were also evaluated. Compared to oxidative damaged mice, SMA increased the activities of SOD, GSH-Px, CAT and T-AOC but decreased the levels of MDA in the serum and liver. SMA significantly changed the composition and diversity of the gut microbiota. Specifically, SMA increased the relative abundance of the phylum Firmicutes and decreased the relative abundance of the phyla Bacteroidetes. At the genus level, SMA significantly increased the relative abundance of Lactobacillus, but decreased the relative abundance of Bacteroides. In addition, SMA also reversed carbohydrate metabolism and amino acid metabolism to normal levels. It indicates that SMA could protect the body from oxidative damage and be used as a potential functional food to prevent diseases related to oxidative stress. PRACTICAL APPLICATIONS: Anthocyanins provide protective effects against harmful effect of oxidative stress. Natural anthocyanins are safer and nutritious as compared to synthetic pigments. However, their stability is poor. The previous research done by this group showed that the anthocyanins content of variety of Vitis amurensis Rupr was as high as 180 mg/(100 g·FW), and the content of malvids anthocyanidin in its ingredients was the highest of all. Malvids anthocyanin and caffeic acid are bonded to produce stabilized malvids anthocyanins (SMA) high hydrostatic pressure technology, which has better stability. Our results indicate that SMA could increased the activities of antioxidant enzymes and altered the composition and diversity of the gut microbiota in mice with oxidative damage. The study will help to deepen the understanding of antioxidative stress mechanism of SMA and lay a foundation for the application of natural anthocyanidin in health aspect.
矢车菊素类花色苷已被证明具有显著的抗氧化活性。然而，由于天然花色苷容易受到温度、光照和 pH 值的影响，因此其稳定性较差。它们可以与咖啡酸产生共色素化作用，从而提高颜色稳定性。本研究旨在调查体内稳定矢车菊素类花色苷（SMA）的抗氧化应激功能作用。研究了 SMA 对氧化损伤小鼠血清和肝脏中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、过氧化氢酶（CAT）、总抗氧化能力（T-AOC）和丙二醛（MDA）的变化。还评估了 SMA 对氧化损伤小鼠肠道微生物多样性的影响。与氧化损伤小鼠相比，SMA 增加了 SOD、GSH-Px、CAT 和 T-AOC 的活性，但降低了血清和肝脏中 MDA 的水平。SMA 显著改变了肠道微生物群的组成和多样性。具体来说，SMA 增加了厚壁菌门的相对丰度，降低了拟杆菌门的相对丰度。在属水平上，SMA 显著增加了乳杆菌属的相对丰度，但降低了拟杆菌属的相对丰度。此外，SMA 还使碳水化合物代谢和氨基酸代谢恢复到正常水平。这表明 SMA 可以保护身体免受氧化损伤，并可用作预防与氧化应激相关疾病的潜在功能性食品。
花色苷对氧化应激的有害影响具有保护作用。与合成色素相比，天然花色苷更安全、更有营养。然而，它们的稳定性较差。本研究小组之前的研究表明，北五味子的花色苷含量高达 180mg/（100g·FW），其成分中矢车菊素的含量最高。矢车菊素和咖啡酸结合产生稳定的矢车菊素（SMA），然后用高静压技术处理，使其具有更好的稳定性。我们的结果表明，SMA 可以提高抗氧化酶的活性，并改变氧化损伤小鼠肠道微生物群的组成和多样性。该研究将有助于深化对 SMA 抗氧化应激机制的认识，并为天然花色苷在健康方面的应用奠定基础。
