Walsh B J, Baldo B A, Bass D J, Clancy R, Musk A W, Wrigley C W
N Engl Reg Allergy Proc. 1987 Jan-Feb;8(1):27-33. doi: 10.2500/108854187779045376.
The need for better in vitro testing for wheat allergy particularly involved correlating clinical evidence of Type I hypersensitivity with laboratory detection of specific IgE antibodies in serum. We report here an improvement in this relationship by the use of a modified method for RAST (Radioallergosorbent test), involving nitrocellulose as the solid phase and alkali (or ethanol) for extraction of allergens and treatment of discs. Serum IgE reactions with the full range of wheat grain and dust proteins were studied using this method and the results were related to wheat allergies due to flour ingestion and the inhalation of flour, pollen or grain dust.
对小麦过敏进行更好的体外检测的需求，尤其涉及将I型超敏反应的临床证据与血清中特异性IgE抗体的实验室检测相关联。我们在此报告，通过使用一种改良的RAST（放射变应原吸附试验）方法，这种关系得到了改善，该方法涉及使用硝酸纤维素作为固相，并用碱（或乙醇）提取过敏原和处理圆盘。使用该方法研究了血清IgE与全系列小麦籽粒和粉尘蛋白的反应，结果与因摄入面粉以及吸入面粉、花粉或谷物粉尘引起的小麦过敏相关。
