Walsh B J, Wrigley C W, Musk A W, Baldo B A
J Allergy Clin Immunol. 1985 Jul;76(1):23-8. doi: 10.1016/0091-6749(85)90799-7.
The IgE-binding proteins from flour, associated with bakers' asthma, have been reassessed by use of a modified RAST suitable for both soluble and insoluble proteins. Nitrocellulose sheet was used for preparing RAST discs, and seven different solvents were compared for their suitability in preparing discs. Dilute alkali (1% potassium hydroxide) was chosen as the best solvent for disc preparation, and its use was compared with that of water as solvent. RAST analyses of sera from 24 allergic bakers demonstrated that the albumin fraction of flour is clearly allergenic (as found in previous studies), but in addition, major IgE-binding proteins were found in the other three fractions (globulin, gliadin, and glutenin) when potassium hydroxide was the solvent (but not with water). We conclude that current RAST procedures, which favor water-soluble allergens, are inadequate because they do not satisfactorily test for water-insoluble allergens.
已使用一种适用于可溶性和不溶性蛋白质的改良放射变应原吸附试验（RAST），对与面包师哮喘相关的面粉中的IgE结合蛋白进行了重新评估。用硝酸纤维素膜制备RAST圆盘，并比较了七种不同溶剂制备圆盘的适用性。稀碱（1%氢氧化钾）被选为制备圆盘的最佳溶剂，并将其与用水作为溶剂的情况进行了比较。对24名过敏性面包师的血清进行的RAST分析表明，面粉的白蛋白部分明显具有致敏性（如先前研究中所发现），但此外，当氢氧化钾作为溶剂时（用水作为溶剂时未发现），在其他三个部分（球蛋白、醇溶蛋白和谷蛋白）中发现了主要的IgE结合蛋白。我们得出结论，目前倾向于检测水溶性过敏原的RAST方法并不充分，因为它们不能令人满意地检测水不溶性过敏原。
