迈向对核糖核酸酶A折叠的理解。
Toward an understanding of the folding of ribonuclease A.
作者信息
Scheraga H A, Konishi Y, Rothwarf D M, Mui P W
出版信息
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5740-4. doi: 10.1073/pnas.84.16.5740.
Abstract
A mechanism was proposed several years ago for the regeneration of native ribonuclease A (EC 3.1.27.5) from the fully reduced form by a mixture of oxidized and reduced glutathiones. Several folding pathways, depending on the solution conditions, were deduced. It is shown here that recent criticisms of those results are due to a misinterpretation of the analysis of our data. A more detailed description of our method of analysis of our previous kinetic and energetic data is presented in order to clarify possible misconceptions.
摘要
几年前有人提出了一种机制，用于通过氧化型和还原型谷胱甘肽的混合物从完全还原形式再生天然核糖核酸酶A（EC 3.1.27.5）。根据溶液条件推导了几种折叠途径。本文表明，最近对这些结果的批评是由于对我们数据的分析存在误解。为了澄清可能存在的误解，本文对我们之前动力学和能量数据的分析方法进行了更详细的描述。
相似文献
1
Toward an understanding of the folding of ribonuclease A.迈向对核糖核酸酶A折叠的理解。
Proc Natl Acad Sci U S A. 1987 Aug;84(16):5740-4. doi: 10.1073/pnas.84.16.5740.
2
Regeneration of bovine pancreatic ribonuclease A: detailed kinetic analysis of two independent folding pathways.牛胰核糖核酸酶A的再生：两条独立折叠途径的详细动力学分析
Biochemistry. 1998 Mar 17;37(11):3767-76. doi: 10.1021/bi972823f.
3
Kinetic folding pathway of a three-disulfide mutant of bovine pancreatic ribonuclease A missing the [40-95] disulfide bond.缺失[40-95]二硫键的牛胰核糖核酸酶A的三二硫键突变体的动力学折叠途径。
Biochemistry. 1998 May 19;37(20):7561-71. doi: 10.1021/bi980086x.
4
Further experimental studies of the disulfide folding transition of ribonuclease A.核糖核酸酶A二硫键折叠转变的进一步实验研究。
Proteins. 1988;4(4):251-61. doi: 10.1002/prot.340040404.
5
Toward a better understanding of protein folding pathways.迈向对蛋白质折叠途径的更好理解。
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5082-6. doi: 10.1073/pnas.85.14.5082.
6
Catalysis of the oxidative folding of bovine pancreatic ribonuclease A by protein disulfide isomerase.蛋白质二硫键异构酶催化牛胰核糖核酸酶A的氧化折叠。
J Mol Biol. 2000 Jul 21;300(4):995-1003. doi: 10.1006/jmbi.2000.3928.
7
Regeneration of three-disulfide mutants of bovine pancreatic ribonuclease A missing the 65-72 disulfide bond: characterization of a minor folding pathway of ribonuclease A and kinetic roles of Cys65 and Cys72.缺失65-72二硫键的牛胰核糖核酸酶A的三二硫键突变体的再生：核糖核酸酶A一条次要折叠途径的表征以及半胱氨酸65和半胱氨酸72的动力学作用
Biochemistry. 1998 Mar 31;37(13):4490-501. doi: 10.1021/bi9725327.
8
Role of two proline-containing turns in the folding of porcine ribonuclease.两个含脯氨酸的转角在猪核糖核酸酶折叠中的作用。
J Mol Biol. 1990 Mar 5;212(1):185-96. doi: 10.1016/0022-2836(90)90314-C.
9
Regeneration of RNase A from the reduced protein: models of regeneration pathways.还原型核糖核酸酶A的再生：再生途径模型
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5734-8. doi: 10.1073/pnas.79.18.5734.
10
Asymmetric kinetics of protein structural changes.蛋白质结构变化的不对称动力学
Acc Chem Res. 2009 Jun 16;42(6):778-87. doi: 10.1021/ar800266r.
引用本文的文献
1
Revisiting the Formation of a Native Disulfide Bond: Consequences for Protein Regeneration and Beyond.重新审视天然二硫键的形成：对蛋白质再生及其他方面的影响。
Molecules. 2020 Nov 16;25(22):5337. doi: 10.3390/molecules25225337.
2
My 65 years in protein chemistry.我在蛋白质化学领域的65年。
Q Rev Biophys. 2015 May;48(2):117-77. doi: 10.1017/S0033583514000134. Epub 2015 Apr 8.
3
Site-specific S-glutathiolation of mitochondrial NADH ubiquinone reductase.线粒体NADH泛醌还原酶的位点特异性S-谷胱甘肽化
Biochemistry. 2007 May 15;46(19):5754-65. doi: 10.1021/bi602580c. Epub 2007 Apr 20.
4
Definition of amide protection factors for early kinetic intermediates in protein folding.蛋白质折叠早期动力学中间体的酰胺保护因子的定义。
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4299-302. doi: 10.1073/pnas.95.8.4299.
5
Expression of wild-type and mutant bovine pancreatic ribonuclease A in Escherichia coli.
Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):615-9. doi: 10.1073/pnas.90.2.615.
6
Local interactions favor the native 8-residue disulfide loop in the oxidation of a fragment corresponding to the sequence Ser-50-Met-79 derived from bovine pancreatic ribonuclease A.在对源自牛胰核糖核酸酶A、对应于序列Ser-50-Met-79的片段进行氧化时，局部相互作用有利于形成天然的8个残基的二硫键环。
J Protein Chem. 1988 Aug;7(4):377-98. doi: 10.1007/BF01024887.
7
Toward a better understanding of protein folding pathways.迈向对蛋白质折叠途径的更好理解。
Proc Natl Acad Sci U S A. 1988 Jul;85(14):5082-6. doi: 10.1073/pnas.85.14.5082.
8
Protein folding/refolding analysis by mass spectrometry. Scrambling of disulphide bridges in insulin.通过质谱法进行蛋白质折叠/重折叠分析。胰岛素中二硫键的错配。
Biochem J. 1990 Jun 15;268(3):803-6. doi: 10.1042/bj2680803.
本文引用的文献
1
Ultraviolet difference spectroscopy of intermediates trapped in unfolding and refolding of bovine pancreatic trypsin inhibitor.
Biochemistry. 1980 Oct 14;19(21):4936-44. doi: 10.1021/bi00562a037.
2
Regeneration of ribonuclease A from the reduced protein. 2. Conformational analysis of the intermediates by nuclear magnetic resonance spectroscopy.还原型核糖核酸酶A的再生。2. 通过核磁共振光谱对中间体进行构象分析。
Biochemistry. 1980 Apr 1;19(7):1316-22. doi: 10.1021/bi00548a009.
3
Regeneration of ribonuclease A from the reduced protein. 1. Conformational analysis of the intermediates by measurements of enzymatic activity, optical density, and optical rotation.
Biochemistry. 1980 Apr 1;19(7):1308-16. doi: 10.1021/bi00548a008.
4
Circular dichroism, Raman spectroscopy, and gel filtration of trapped folding intermediates of ribonuclease.核糖核酸酶捕获折叠中间体的圆二色性、拉曼光谱和凝胶过滤
Biochemistry. 1981 Feb 3;20(3):594-601. doi: 10.1021/bi00506a023.
5
Regeneration of ribonuclease A from the reduced protein. Energetic analysis.
Biochemistry. 1982 Sep 14;21(19):4741-8. doi: 10.1021/bi00262a034.
6
Regeneration of ribonuclease A from the reduced protein. Rate-limiting steps.
Biochemistry. 1982 Sep 14;21(19):4734-40. doi: 10.1021/bi00262a033.
7
Regeneration of RNase A from the reduced protein: models of regeneration pathways.还原型核糖核酸酶A的再生：再生途径模型
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5734-8. doi: 10.1073/pnas.79.18.5734.
8
Mechanism for the unfolding and refolding of ribonuclease A. Kinetic studies utilizing spectroscopic methods.核糖核酸酶A的去折叠和重折叠机制。利用光谱方法进行的动力学研究。
Biochemistry. 1983 Feb 1;22(3):564-73. doi: 10.1021/bi00272a007.
9
Disulfide bond formation in proteins.蛋白质中二硫键的形成。
Methods Enzymol. 1984;107:305-29. doi: 10.1016/0076-6879(84)07021-x.
10
Involvement of prolines-114 and -117 in the slow refolding phase of ribonuclease A as determined by isomer-specific proteolysis.通过异构体特异性蛋白水解确定脯氨酸-114和-117参与核糖核酸酶A的缓慢重折叠阶段。
Biochemistry. 1984 Nov 20;23(24):5713-23. doi: 10.1021/bi00319a009.
Zotero 插件