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迈向对核糖核酸酶A折叠的理解。

Toward an understanding of the folding of ribonuclease A.

作者信息

Scheraga H A, Konishi Y, Rothwarf D M, Mui P W

出版信息

Proc Natl Acad Sci U S A. 1987 Aug;84(16):5740-4. doi: 10.1073/pnas.84.16.5740.

DOI:10.1073/pnas.84.16.5740
PMID:3475701
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC298938/
Abstract

A mechanism was proposed several years ago for the regeneration of native ribonuclease A (EC 3.1.27.5) from the fully reduced form by a mixture of oxidized and reduced glutathiones. Several folding pathways, depending on the solution conditions, were deduced. It is shown here that recent criticisms of those results are due to a misinterpretation of the analysis of our data. A more detailed description of our method of analysis of our previous kinetic and energetic data is presented in order to clarify possible misconceptions.

摘要

几年前有人提出了一种机制，用于通过氧化型和还原型谷胱甘肽的混合物从完全还原形式再生天然核糖核酸酶A（EC 3.1.27.5）。根据溶液条件推导了几种折叠途径。本文表明，最近对这些结果的批评是由于对我们数据的分析存在误解。为了澄清可能存在的误解，本文对我们之前动力学和能量数据的分析方法进行了更详细的描述。

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Local interactions favor the native 8-residue disulfide loop in the oxidation of a fragment corresponding to the sequence Ser-50-Met-79 derived from bovine pancreatic ribonuclease A.在对源自牛胰核糖核酸酶A、对应于序列Ser-50-Met-79的片段进行氧化时，局部相互作用有利于形成天然的8个残基的二硫键环。
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本文引用的文献

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Ultraviolet difference spectroscopy of intermediates trapped in unfolding and refolding of bovine pancreatic trypsin inhibitor.
Biochemistry. 1980 Oct 14;19(21):4936-44. doi: 10.1021/bi00562a037.
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Regeneration of ribonuclease A from the reduced protein. 2. Conformational analysis of the intermediates by nuclear magnetic resonance spectroscopy.还原型核糖核酸酶A的再生。2. 通过核磁共振光谱对中间体进行构象分析。
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Circular dichroism, Raman spectroscopy, and gel filtration of trapped folding intermediates of ribonuclease.核糖核酸酶捕获折叠中间体的圆二色性、拉曼光谱和凝胶过滤
Biochemistry. 1981 Feb 3;20(3):594-601. doi: 10.1021/bi00506a023.
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Regeneration of ribonuclease A from the reduced protein. Energetic analysis.
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Regeneration of ribonuclease A from the reduced protein. Rate-limiting steps.
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Regeneration of RNase A from the reduced protein: models of regeneration pathways.还原型核糖核酸酶A的再生：再生途径模型
Proc Natl Acad Sci U S A. 1982 Sep;79(18):5734-8. doi: 10.1073/pnas.79.18.5734.
8
Mechanism for the unfolding and refolding of ribonuclease A. Kinetic studies utilizing spectroscopic methods.核糖核酸酶A的去折叠和重折叠机制。利用光谱方法进行的动力学研究。
Biochemistry. 1983 Feb 1;22(3):564-73. doi: 10.1021/bi00272a007.
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Disulfide bond formation in proteins.蛋白质中二硫键的形成。
Methods Enzymol. 1984;107:305-29. doi: 10.1016/0076-6879(84)07021-x.
10
Involvement of prolines-114 and -117 in the slow refolding phase of ribonuclease A as determined by isomer-specific proteolysis.通过异构体特异性蛋白水解确定脯氨酸-114和-117参与核糖核酸酶A的缓慢重折叠阶段。
Biochemistry. 1984 Nov 20;23(24):5713-23. doi: 10.1021/bi00319a009.