Jang Yu-Sin, Seong Hyeon Jeong, Kwon Seong Woo, Lee Yong-Suk, Im Jung Ae, Lee Haeng Lim, Yoon Ye Rin, Lee Sang Yup
Division of Applied Life Science (BK21), Department of Applied Life Chemistry, Institute of Agriculture and Life Science (IALS), Gyeongsang National University, Jinju, South Korea.
Department of Chemical and Biomolecular Engineering (BK21 Plus Program), BioProcess Engineering Research Center, Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, South Korea.
Front Bioeng Biotechnol. 2021 Oct 25;9:754250. doi: 10.3389/fbioe.2021.754250. eCollection 2021.
ATPase, a key enzyme involved in energy metabolism, has not yet been well studied in . Here, we knocked down the gene encoding the ATPase gamma subunit in ATCC 824 using a mobile group II intron system and analyzed the physiological characteristics of the gene knockdown mutant, 824-2866KD. Properties investigated included cell growth, glucose consumption, production of major metabolites, and extracellular pH. Interestingly, in 2-L batch fermentations, 824-2866KD showed no significant difference in metabolite biosynthesis or cell growth compared with the parent ATCC 824. However, the pH value in 824-2866KD cultures at the late stage of the solventogenic phase was abnormally high (pH 6.12), compared with that obtained routinely in the culture of ATCC 824 (pH 5.74). This phenomenon was also observed in batch cultures of another , BEKW-2866KD, an -knockdown and double-knockout mutant. The findings reported in this study suggested that ATPase is relatively minor than acid-forming pathway in ATP metabolism in
ATP酶是参与能量代谢的关键酶，在……中尚未得到充分研究。在此，我们使用II组内含子移动系统敲低了ATCC 824中编码ATP酶γ亚基的基因，并分析了该基因敲除突变体824 - 2866KD的生理特性。研究的特性包括细胞生长、葡萄糖消耗、主要代谢产物的产生以及细胞外pH值。有趣的是，在2升分批发酵中，与亲本ATCC 824相比，824 - 2866KD在代谢物生物合成或细胞生长方面没有显著差异。然而，与ATCC 824培养中常规获得的pH值（pH 5.74）相比，824 - 2866KD培养物在产溶剂阶段后期的pH值异常高（pH 6.12）。在另一个……的分批培养中，即敲低……和……双敲除突变体BEKW - 2866KD中也观察到了这种现象。本研究报告的结果表明，在……的ATP代谢中，ATP酶相对于产酸途径来说相对次要。
