Synthetic Biology Tools for Genome and Transcriptome Engineering of Solventogenic .

Strains of genus are used for production of various value-added products including fuels and chemicals. Development of any commercially viable production process requires a combination of both strain and fermentation process development strategies. The strain development in sp. could be achieved by random mutagenesis, and targeted gene alteration methods. However, strain improvement in sp. by targeted gene alteration method was challenging due to the lack of efficient tools for genome and transcriptome engineering in this organism. Recently, various synthetic biology tools have been developed to facilitate the strain engineering of solventogenic . In this review, we consolidated the recent advancements in toolbox development for genome and transcriptome engineering in solventogenic . Here we reviewed the genome-engineering tools employing mobile group II intron, alleles exchange, and CRISPR/Cas9 with their application for strain development of sp. Next, transcriptome engineering tools such as untranslated region (UTR) engineering and synthetic sRNA techniques were also discussed in context of strain engineering. Application of any of these discussed techniques will facilitate the metabolic engineering of clostridia for development of improved strains with respect to requisite functional attributes. This might lead to the development of an economically viable butanol production process with improved titer, yield and productivity.