Biochemistry and Biotechnology Laboratory, Central Leather Research Institute, Council of Scientific and Industrial Research (CSIR), Adyar, Chennai, 600020, India.
Department of Leather Technology, Housed at CSIR-Central Leather Research Institute, Alagappa College of Technology, Anna University, Chennai, 600020, India.
Appl Biochem Biotechnol. 2022 Jan;194(1):302-322. doi: 10.1007/s12010-021-03741-3. Epub 2021 Nov 11.
The most important role of tissue engineering is to develop a biomaterial with a property that mimics the extracellular matrix (ECM) by enhancing the lineage-specific proliferation and differentiation with favorable regeneration property to aid in new tissue formation. Thus, to develop an ideal scaffold for bone repair, we have fabricated a composite nanofiber by the coaxial electrospinning technique. The coaxial electrospun nanofiber contains the core layer, consisting of polyvinyl alcohol (PVA) blended with oregano extract and mesoporous silica nanoparticles (PVA-OE-MSNPs), and the shell layer, consisting of poly-ε-caprolactone blended with collagen and hydroxyapatite (PCL-collagen-HAP). We evaluated the physicochemical properties of the nanofibers using X-ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and Fourier transform infrared spectroscopy (FTIR). In vitro biocompatibility, cell adhesion, cell viability, and osteogenic potential were evaluated by 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenlytetrazolium bromide (MTT), calcein AM, and alkaline phosphatase (ALP) activity and Alizarin Red staining in NIH 3T3/MG-63 cells. The results showed that the nanoparticle-incorporated coaxial nanofiber was observed with bead-free, continuous, and uniform fiber morphology with a mean diameter in the range of 310 ± 125 nm. From the biochemical studies, it is observed that the incorporation of nanofiber with HAP and MSNPs shows good swelling property with ideal porosity, biodegradation, and enhanced biomineralization property. In vitro results showed that the scaffolds with nanoparticles have higher cell adhesion, cell viability, ALP activity, and mineralization potential. Thus, the fabricated nanofiber could be an appropriate implantable biomaterial for bone tissue engineering.
组织工程的最重要作用是开发一种具有模仿细胞外基质（ECM）特性的生物材料，通过增强谱系特异性增殖和分化，同时具有有利的再生特性，从而有助于新组织的形成。因此，为了开发用于骨修复的理想支架，我们通过同轴静电纺丝技术制备了一种复合纳米纤维。同轴电纺纳米纤维包含芯层，由聚（乙醇酸）（PVA）与牛至提取物和介孔硅纳米粒子（PVA-OE-MSNPs）共混组成，以及壳层，由聚己内酯（PCL）与胶原蛋白和羟基磷灰石（PCL-胶原蛋白-HAP）共混组成。我们使用 X 射线衍射（XRD）、热重分析（TGA）、扫描电子显微镜（SEM）和傅里叶变换红外光谱（FTIR）评估了纳米纤维的物理化学性质。通过 3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四氮唑溴盐（MTT）、钙黄绿素 AM 和碱性磷酸酶（ALP）活性和茜素红染色评估纳米纤维的体外生物相容性、细胞黏附、细胞活力和成骨潜力在 NIH 3T3/MG-63 细胞中。结果表明，观察到纳米颗粒掺入的同轴纳米纤维具有无珠、连续和均匀的纤维形态，平均直径在 310±125nm 范围内。从生化研究中可以看出，将纳米纤维与 HAP 和 MSNPs 结合使用，具有良好的溶胀性能、理想的孔隙率、生物降解性和增强的生物矿化性能。体外结果表明，具有纳米颗粒的支架具有更高的细胞黏附性、细胞活力、ALP 活性和矿化潜力。因此，所制备的纳米纤维可能是一种合适的用于骨组织工程的可植入生物材料。
