Oral and maxillofacial surgery resident, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Marquette University School of Dentistry, Milwaukee, WI, USA.
Mater Sci Eng C Mater Biol Appl. 2019 Jul;100:564-575. doi: 10.1016/j.msec.2019.03.003. Epub 2019 Mar 5.
Recent exciting findings of the particular properties of Carbon dot (CDs) have shed light on potential biomedical applications of CDs-containing composites. While CDs so far have been widely used as biosensors and bioimaging agents, in the present study for the first time, we evaluate the osteoconductivity of CDs in poly (ε-caprolactone) (PCL)/polyvinyl alcohol (PVA) [PCL/PVA] nanofibrous scaffolds. Moreover, further studies were performed to evaluate egg shell-derived calcium phosphate (TCP3) and its cellular responses, biocompatibility and in vitro osteogenesis. Scaffolds were fabricated by simultaneous electrospinning of PCL with three different types of calcium phosphate, PVA and CDs. Fabricated scaffolds were characterized by Scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), X-ray powder diffraction (XRD), contact angle measurement and degradation assessment. SEM, the methyl thiazolyl tetrazolium (MTT) assay, and alkaline phosphatase (ALP) activity test were performed to evaluate cell morphology, proliferation and osteogenic differentiation, respectively. The results demonstrated that while the addition of just 1 wt% CDs and TCP3 individually into PCL/PVA nanocomposite enhanced ALP activity and cell proliferation rate (p < 0.05), the synergetic effect of CDs/TCP3 led to highest osteogenic differentiation and proliferation rate compared to other scaffolds (p < 0.05). Hence, CDs and PCL/PVA-TCP3 could serve as a potential candidate for bone tissue regeneration.
最近，碳点（CDs）的特殊性质的令人兴奋的发现为含 CD 的复合材料的潜在生物医学应用提供了启示。虽然 CDs 迄今为止已被广泛用作生物传感器和生物成像剂，但在本研究中，我们首次评估了 CDs 在聚（ε-己内酯）（PCL）/聚乙烯醇（PVA）[PCL/PVA]纳米纤维支架中的骨诱导性。此外，还进行了进一步的研究来评估蛋壳衍生的磷酸钙（TCP3）及其细胞反应、生物相容性和体外成骨作用。通过 PCL 与三种不同类型的磷酸钙、PVA 和 CDs 的同时电纺来制备支架。通过扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR）、X 射线粉末衍射（XRD）、接触角测量和降解评估来表征制备的支架。通过 SEM、甲基噻唑基四唑（MTT）测定和碱性磷酸酶（ALP）活性测试分别评估细胞形态、增殖和成骨分化。结果表明，仅添加 1wt% CDs 和 TCP3 分别到 PCL/PVA 纳米复合材料中就可以提高 ALP 活性和细胞增殖率（p<0.05），而 CDs/TCP3 的协同作用与其他支架相比导致了最高的成骨分化和增殖率（p<0.05）。因此，CDs 和 PCL/PVA-TCP3 可用作骨组织再生的潜在候选材料。
