Laboratory of Invertebrate Immunological Defense and Reproductive Biology, School of Life Sciences, East China Normal University, Shanghai, China.
Laboratory of Invertebrate Immunological Defense and Reproductive Biology, School of Life Sciences, East China Normal University, Shanghai, China.
Dev Comp Immunol. 2022 Mar;128:104313. doi: 10.1016/j.dci.2021.104313. Epub 2021 Nov 9.
Down syndrome cell adhesion molecule (Dscam), also called hypervariable Dscam (Dscam-hv), is an important player in arthropod alternative splicing that connects neurons and immune regulation, acting as a pathogen-specific recognition molecule. Dscam-hv has two forms: transmembrane (TM) Dscam (mDscam) and soluble Dscam (sDscam). Herein, we investigated two transmembrane variants of mDscam resulting from alternative splicing of the transmembrane domain, focusing on differences in their immune regulation. We characterized the Dscam[TM1] and Dscam[TM2] genes of Chinese mitten crab (Eriocheir sinensis) through bioinformatics analysis. Both genes are expressed in the gill, intestine, and other immune tissues. Following gram-positive and gram-negative bacteria stimulation, EsDscam[TM1] and EsDscam[TM2] mRNA expression levels increased significantly in hemocytes. Sequencing showed that EsDscam[TM1] was more abundant in hemocytes than EsDscam[TM2]. Additionally, the two subtypes differ in their regulation of antimicrobial peptides, the proportion of exon 33 carried, and bacterial phagocytosis.
Down 综合征细胞黏附分子（Dscam），也称为高变异 Dscam（Dscam-hv），是连接神经元和免疫调节的节肢动物可变剪接的重要参与者，作为一种病原体特异性识别分子。Dscam-hv 有两种形式：跨膜（TM）Dscam（mDscam）和可溶性 Dscam（sDscam）。在此，我们研究了跨膜域可变剪接产生的 mDscam 的两种跨膜变体，重点研究了它们在免疫调节方面的差异。我们通过生物信息学分析对中国绒螯蟹（Eriocheir sinensis）的 Dscam[TM1]和 Dscam[TM2]基因进行了表征。这两个基因在鳃、肠和其他免疫组织中表达。在革兰氏阳性和革兰氏阴性细菌刺激后，血细胞中 EsDscam[TM1]和 EsDscam[TM2]的 mRNA 表达水平显著增加。测序表明，EsDscam[TM1]在血细胞中的丰度高于 EsDscam[TM2]。此外，这两种亚型在抗菌肽的调控、携带外显子 33 的比例和细菌吞噬作用方面存在差异。
