College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, 230031, PR China.
College of Animal Science and Technology, Anhui Agricultural University, Hefei City, Anhui Province, 230031, PR China.
Fish Shellfish Immunol. 2024 Aug;151:109626. doi: 10.1016/j.fsi.2024.109626. Epub 2024 May 24.
In arthropods, the involvement of Dscam (Down syndrome cell adhesion molecule) in innate immunity has been extensively demonstrated. Its cytoplasmic tail contains multiple conserved functional sites, which indicates its involvement in different intracellular signaling pathways. In this study, we focused on the role of the cytoplasmic tail of Dscam in the Chinese mitten crab (Eriocheir sinensis) immune defense. In the group with cytoplasmic tail knockdown (the site was located on constant exons 37 and 38), 3885 differentially expressed genes (DEGs) were identified. The DEGs were enriched in small molecule binding, protein-containing complex binding, and immunity-related pathways. The expression of selected genes were validated using quantitative real-time reverse transcription PCR. We identified key Cell cycle, Janus kinase (JAK)-signal transducer, activator of transcription (STAT) and mitogen-activated protein kinase (MAPK) signaling pathway genes, the results indicated that the cytoplasmic tail of Dscam controls antibacterial responses by regulating cell proliferation-related genes in hemocytes.
在节肢动物中，Dscam（唐氏综合征细胞黏附分子）在先天免疫中的作用已被广泛证实。其细胞质尾部包含多个保守的功能位点，表明其参与了不同的细胞内信号通路。在这项研究中，我们专注于 Dscam 细胞质尾部在中华绒螯蟹（Eriocheir sinensis）免疫防御中的作用。在细胞质尾部敲低组（该位点位于恒定外显子 37 和 38 上）中，鉴定到 3885 个差异表达基因（DEGs）。DEGs 富集于小分子结合、含蛋白质的复合物结合和免疫相关途径。使用定量实时逆转录 PCR 验证了选定基因的表达。我们鉴定了关键的细胞周期、Janus 激酶 (JAK)-信号转导子和转录激活因子 (STAT) 和丝裂原激活蛋白激酶 (MAPK) 信号通路基因，结果表明 Dscam 的细胞质尾部通过调节血细胞中与细胞增殖相关的基因来控制抗菌反应。
