Cytology Diagnostic Services, Institute of Biomedical Sciences Abel Salazar (ICBAS-UP), University of Porto, Porto, Portugal.
Veterinary Clinic, Institute of Biomedical Sciences Abel Salazar (ICBAS-UP), University of Porto, Porto, Portugal.
J Vet Diagn Invest. 2022 Mar;34(2):263-267. doi: 10.1177/10406387211058825. Epub 2021 Nov 11.
Quantitative morphologic parameters assessed in cytologic samples of canine cutaneous mast cell tumors (ccMCTs) may assist with surgical planning and prognostication. Robust cutoffs can be defined, with high reproducibility, for parameters such as the nuclear area (NA). The NA may be determined by morphometry (image analysis, NAI) or by stereology, such as the 2D-nucleator method (NAN); stereologic techniques have not been applied to cytologic specimens of ccMCT, to our knowledge. We retrospectively selected routine cytology smears from 51 ccMCT cases and screened them to determine the percentage of neoplastic mast cells with indistinct nuclear borders; this was repeated after the slides were restained with H&E. The NAI and the NAN were estimated in 100 mast cells per animal in H&E-stained slides. All nuclei were visible in H&E smears, and unbiased quantification was feasible. The NAN was similar to NAI, but less time-consuming. Both the NAN and NAI determined by cytology differed in histologic low- and high-grade ccMCTs, and in histologic grade I plus II versus grade III ccMCTs. Stereologic parameters such as the NAN could be considered as complementary techniques for the cytologic evaluation of ccMCTs.
在犬皮肤肥大细胞瘤（ccMCT）的细胞学样本中评估的定量形态学参数可能有助于手术规划和预后判断。可以定义具有高重复性的参数的稳健截止值，例如核面积（NA）。NA 可以通过形态计量学（图像分析，NAI）或立体学确定，例如 2D 核器法（NAN）；据我们所知，立体学技术尚未应用于 ccMCT 的细胞学标本。我们回顾性地选择了 51 例 ccMCT 常规细胞学涂片，并对其进行筛选，以确定具有不清晰核边界的肿瘤性肥大细胞的百分比；在重新用 H&E 染色后重复此操作。在 H&E 染色的载玻片上，估计每个动物的 100 个肥大细胞的 NAI 和 NAN。H&E 涂片上可以看到所有细胞核，并且可以进行无偏置的定量。NAN 与 NAI 相似，但耗时更少。细胞学确定的 NAN 和 NAI 在组织学低级别和高级别 ccMCT 以及组织学 I 级加 II 级与 III 级 ccMCT 中有所不同。立体学参数，如 NAN，可以被视为 ccMCT 细胞学评估的补充技术。
