Strefezzi Ricardo de Francisco, Xavier José Guilherme, Kleeb Silvia Regina, Catão-Dias José Luiz
Departamento de Patologia, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, State of São Paulo, Brazil.
J Vet Diagn Invest. 2009 Nov;21(6):821-5. doi: 10.1177/104063870902100608.
Twenty-nine canine cutaneous mast cell tumors (MCTs) were morphometrically analyzed with regard to mean nuclear area (MNA) using cytopathology smears. The results showed a correlation between MNA and survival. When graded into 2 morphometrically different groups, there were statistically significant differences among high- and low-grade MCTs, regarding both Romanowsky-type stain and hematoxylin and eosin. Cytomorphometry could also separate histologic grade II tumors with better prognosis from the more aggressive MCTs. The results indicated that nuclear morphometry on cytopathology preparations can predict the biological behavior of cutaneous MCTs in dogs in an independent manner, yielding a rapid and reproducible diagnosis, which renders the method useful for veterinary oncology.
对29例犬皮肤肥大细胞瘤（MCTs）的细胞病理学涂片进行平均核面积（MNA）的形态计量分析。结果显示MNA与生存率之间存在相关性。当分为两个形态计量学不同的组时，无论对于罗曼诺夫斯基型染色还是苏木精和伊红染色，高分级和低分级MCTs之间均存在统计学显著差异。细胞形态计量学还可以将预后较好的组织学II级肿瘤与侵袭性更强的MCTs区分开来。结果表明，细胞病理学制片上的核形态计量学能够独立预测犬皮肤MCTs的生物学行为，得出快速且可重复的诊断结果，这使得该方法对兽医肿瘤学有用。
