几种类固醇代谢物在红细胞增多症小鼠中的协同促红细胞生成测定
Cooperative erythropoietic assay of several steroid metabolites in polycythemic mice.
作者信息
Fisher J W, Adamson J W, Camiscoli J F, Fried W, Gordon A S, Schooley J, Zanjani E
出版信息
Steroids. 1977 Dec;30(6):833-45. doi: 10.1016/s0039-128x(77)80029-9.
A blinded cooperative assay of several androstane and pregnane steroid metabolites has been carried out in order to determine whether 5beta-H derivatives are as active as testosterone in stimulating in vivo erythropoiesis. The steroids tested were: testosterone, 5alpha-dihydrotestosterone, 5beta-dihydrotestosterone, 5beta-pregnane-3,20-dione, 3alpha-dihydroxy-5beta-pregnane-11,20-dione and 3beta-hydroxy-5beta-pregnan-20-one. The incorporation of radioactive iron into newly formed red cells in exhypoxic polycythemic mice was used to compare the effects of the steroids. Testosterone and 5alpha-dihydrotestosterone both produced significant increases in 59Fe incorporation. 5beta-dihydrotestosterone, 5beta-pregnane-3,20-dione, 3alpha-hydroxy-5beta-pregnane-11,20-dione and 3beta-hydroxy-5beta-pregnan-20-one were all devoid of significant erythropoietic activity in polycythemic mice in almost all instances. Thus, under the conditions chosen, this study failed to demonstrate that 5beta-steroids increase radioactive iron incorporation in red cells of exhypoxic polycythemic mice.
为了确定5β-H衍生物在刺激体内红细胞生成方面是否与睾酮具有相同的活性，我们对几种雄甾烷和孕甾烷类固醇代谢物进行了一项双盲合作试验。所测试的类固醇包括：睾酮、5α-二氢睾酮、5β-二氢睾酮、5β-孕烷-3,20-二酮、3α-二羟基-5β-孕烷-11,20-二酮和3β-羟基-5β-孕烷-20-酮。通过检测低氧性红细胞增多症小鼠新生成红细胞中放射性铁的掺入情况，来比较这些类固醇的作用。睾酮和5α-二氢睾酮均使59Fe掺入量显著增加。在几乎所有情况下，5β-二氢睾酮、5β-孕烷-3,20-二酮、3α-羟基-5β-孕烷-11,20-二酮和3β-羟基-5β-孕烷-20-酮在红细胞增多症小鼠中均无显著的促红细胞生成活性。因此，在所选条件下，本研究未能证明5β-类固醇能增加低氧性红细胞增多症小鼠红细胞中放射性铁的掺入量。
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