Department of Pharmacy, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, 21000, Novi Sad, Serbia.
Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Hajduk Veljkova 3, Novi Sad, Serbia.
Sci Rep. 2021 Nov 11;11(1):22123. doi: 10.1038/s41598-021-01713-5.
Microencapsulation represents a process that can create targeted, controlled release kinetics of drugs, thus optimizing therapeutic efficacy. Our group has investigated the impact of this technology on Wistar rats to determine pharmacological efficacy of basil extracts. Animals were treated with water extract of Ocimum basilicum in microvesicles and with combination of basil extracts and 3α,7α-dihydroxy-12-keto-5-cholanate, also known as 12-monoketocholic acid (MKC) acid in microvesicles for 7 days. Alloxan was used to induce hyperglycemia. Pharmacological effects on glycemia were evaluated by measuring blood glucose levels in alloxan-induced diabetic rats. Microvesicles were prepared using the Büchi-based microencapsulating system developed in our lab. The dose of basil extract that was orally administered in rats was 200 mg/kg and the dose of MKC acid was 4 mg/kg as per established protocols. A seven-day treatment with basil aqueous extract, as well as a combination of basil and MKC acid extract in the pharmaceutical formulation, led to a statistically significant reduction in the blood glucose concentration of animals with alloxan-induced hyperglycemia compared to pre-treatment values (p < 0.05 and p < 0.01), which indicates that basil has hypoglycemic and antihyperglycemic effects. Microvesicles, as a pharmaceutical-technological formulation, substantially enhance the hypolipidemic action of basil extract with MKC acid.
微胶囊化是一种可以创造靶向、控制药物释放动力学的过程，从而优化治疗效果。我们的团队研究了这项技术对 Wistar 大鼠的影响，以确定罗勒提取物的药理学功效。动物用微囊中的罗勒水提取物和罗勒提取物与 3α,7α-二羟基-12-酮-5-胆酸（也称为 12-单酮胆酸，MKC 酸）的混合物处理 7 天。用链脲佐菌素诱导高血糖。通过测量链脲佐菌素诱导的糖尿病大鼠的血糖水平来评估对血糖的药理作用。微囊使用我们实验室开发的基于 Büchi 的微囊化系统制备。按照既定方案，大鼠口服罗勒提取物的剂量为 200mg/kg，MKC 酸的剂量为 4mg/kg。用罗勒水提取物以及罗勒和 MKC 酸提取物的组合进行为期 7 天的治疗，与治疗前相比，可显著降低链脲佐菌素诱导的高血糖动物的血糖浓度（p<0.05 和 p<0.01），这表明罗勒具有降血糖和抗高血糖作用。微囊作为一种药物技术制剂，大大增强了含有 MKC 酸的罗勒提取物的降血脂作用。
