Hansen T R, Randel R D, Segerson E C, Rutter L M, Harms P G
J Anim Sci. 1987 Aug;65(2):524-33. doi: 10.2527/jas1987.652524x.
Corpus luteum (CL) function following spontaneous or prostaglandin F2 alpha-induced estrus was studied in 27 Brahman cows and 16 Brahman heifers. Females received one injection of 0, .38, .75 or 2.25 mg alfaprostol (PGF)/100 kg body weight at 12 +/- .1 d post-estrus. Serum progesterone (P4) during CL formation, following the subsequent estrus, increased over time (P less than .001, 1 to 13 d post-estrus) and was influenced by PGF (P less than .06) and a PGF X time interaction (P less than .02). Low serum P4 concentrations were observed during CL formation (d 3, 4, 10, and 12) in cattle that received 2.25 mg PGF. On d 13 post-estrus, CL were removed. No differences in weight, P4 content or number of luteinizing hormone (LH) receptors was evident between CL derived 13 d following spontaneous vs PGF-induced estrus. The CL formed following PGF-induced estrus had fewer large (P less than .002) and small (P less than .09) luteal cells in heifers, a lower (P less than .008) percentage composition of histological type I and II luteal cells in cows and a lower (P less than .0001) in vitro P4 response to LH in both cows and heifers when compared with the CL formed following spontaneous estrus. Cows had heavier (P less than .002) CL with a higher (P less than .05) number of large cells, a higher (P less than .09) percentage composition of histological cell type I and II luteal cells, and a higher (P less than .03) in vitro basal and stimulated P4 secretion. It is concluded that 1) the events associated with artificially shortening the estrous cycle with PGF altered subsequent CL function in Brahman females; 2) cows had heavier CL, which were composed of more steroidogenically functional luteal cells, than heifers and 3) age of animal interacted with PGF to alter subsequent CL function in Brahman females.
对27头婆罗门母牛和16头婆罗门小母牛在自然发情或前列腺素F2α诱导发情后黄体（CL）的功能进行了研究。雌性动物在发情后12±0.1天接受一次注射，剂量为0、0.38、0.75或2.25毫克阿法前列醇（PGF）/100千克体重。在黄体形成期间、随后发情之后，血清孕酮（P4）随时间增加（发情后1至13天，P<0.001），并受PGF影响（P<0.06）以及PGF与时间的交互作用影响（P<0.02）。在接受2.25毫克PGF的牛的黄体形成期间（第3、4、10和12天）观察到低血清P4浓度。在发情后第13天，取出黄体。在自然发情与PGF诱导发情后13天形成的黄体之间，黄体重量、P4含量或促黄体生成素（LH）受体数量没有明显差异。与自然发情后形成的黄体相比，PGF诱导发情后形成的黄体在小母牛中大型（P<0.002）和小型（P<0.09）黄体细胞较少，在母牛中组织学I型和II型黄体细胞的百分比组成较低（P<0.008），并且在体外对LH的P4反应在母牛和小母牛中均较低（P<0.0001）。母牛的黄体较重（P<0.002），大型细胞数量较多（P<0.05），组织学细胞I型和II型黄体细胞的百分比组成较高（P<0.09），体外基础和刺激后的P4分泌较高（P<0.03）。得出的结论是：1）与用PGF人为缩短发情周期相关的事件改变了婆罗门雌性动物随后的黄体功能；2）母牛的黄体比小母牛重，且由更多具有类固醇生成功能的黄体细胞组成；3）动物年龄与PGF相互作用，改变了婆罗门雌性动物随后的黄体功能。
