Center of Ultrasound in Medicine, Northwest Women and Children's Hospital, Xi'an, China.
Department of Clinical Laboratory, Northwest Women and Children's Hospital, Xi'an, China.
Comput Math Methods Med. 2021 Nov 2;2021:7145487. doi: 10.1155/2021/7145487. eCollection 2021.
Traditional mass spectrometry detection methods have low detection efficiency for low-abundance proteins, thus limiting the application of proteomic analysis in the diagnosis of preeclampsia. Magnetic nanomaterials have good superparamagnetism and have obvious advantages in the field of biological separation and enrichment.
The objective of this study is to explore the value of superparamagnetic iron oxide nanoparticles in the proteomic analysis of preeclampsia.
42 patients and 40 normal pregnant women were selected in this study for analysis. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were performed to evaluate the function of these differential proteins. Proteomic analysis was used to analyze the differential proteins. Color Doppler ultrasound technology was used to detect changes in the blood flow of the fetal umbilical artery and cerebral artery.
16 differential proteins in the serum of pregnant women with preeclampsia and normal pregnant women were detected. The 16 proteins are mainly related to angiogenesis and endothelial function proteins, coagulation cascade proteins, placental growth factor, and so on. Biological function analysis revealed that these proteins are mainly enriched in the nuclear factor kB (NF-B) signaling pathway. Moreover, our data suggested that compared with the fetus in the uterus of normal pregnant women, the umbilical artery S/D, PI, and RI of the fetus in preeclampsia were greatly increased, and the cerebral artery S/D, PI, and RI were greatly decreased.
Biological function analysis revealed that 16 proteins are mainly enriched in the NF-B signaling pathway. Compared with the normal group, the umbilical artery S/D, PI, and RI of the preeclampsia group were greatly increased, and the cerebral artery S/D, PI, and RI were all greatly reduced. Our findings provided a more comprehensive reference for us to study the mechanism of preeclampsia at the molecular level and also provide data support for the screening of relevant markers for early diagnosis of preeclampsia.
传统的质谱检测方法对低丰度蛋白质的检测效率较低，限制了蛋白质组学分析在子痫前期诊断中的应用。磁性纳米材料具有良好的超顺磁性，在生物分离和富集领域具有明显优势。
本研究旨在探讨超顺磁性氧化铁纳米粒子在子痫前期蛋白质组学分析中的价值。
本研究选取 42 例子痫前期患者和 40 例正常妊娠妇女进行分析。采用基因本体论富集分析和京都基因与基因组百科全书（KEGG）富集分析评估这些差异蛋白的功能。采用蛋白质组学分析技术分析差异蛋白。采用彩色多普勒超声技术检测胎儿脐动脉和大脑中动脉血流变化。
检测到子痫前期孕妇和正常孕妇血清中的 16 个差异蛋白。这 16 种蛋白主要与血管生成和内皮功能蛋白、凝血级联蛋白、胎盘生长因子等有关。生物学功能分析表明，这些蛋白主要富集于核因子 kB（NF-B）信号通路。此外，我们的数据表明，与正常孕妇子宫内胎儿相比，子痫前期胎儿脐动脉 S/D、PI 和 RI 显著升高，大脑中动脉 S/D、PI 和 RI 显著降低。
生物学功能分析表明，16 种蛋白主要富集于 NF-B 信号通路。与正常组相比，子痫前期组脐动脉 S/D、PI 和 RI 显著升高，大脑中动脉 S/D、PI 和 RI 均显著降低。我们的研究结果为我们从分子水平更全面地研究子痫前期的发病机制提供了参考，也为子痫前期早期诊断相关标志物的筛选提供了数据支持。
