Dingwoke Emeka John, Adamude Fatima Amin, Mohamed Gadija, Klein Ashwil, Salihu Aliyu, Abubakar Mujitaba Suleiman, Sallau Abdullahi Balarabe
Department of Biochemistry, Faculty of Life Sciences, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Venom, Antivenom and Natural Toxins Research Centre, Ahmadu Bello University, Zaria, Kaduna State, Nigeria.
Biochem Biophys Rep. 2021 Nov 2;28:101164. doi: 10.1016/j.bbrep.2021.101164. eCollection 2021 Dec.
Snakebite envenoming remains a neglected tropical disease which poses severe health hazard, especially for the rural inhabitants in Africa. In Nigeria, vipers are responsible for the highest number of deaths. Hydrophilic interaction liquid chromatography coupled with LC-MS/MS was used to analyze the crude venoms of (Carpet viper) and (Puff adder) in order to understand their venom proteomic identities. Results obtained revealed that gel-free proteomic analysis of the crude venoms led to the identification of 85 and 79 proteins, respectively. Seventy-eight (78) proteins were common between the two snake species with a 91.8% similarity score. The identified proteins belong to 18 protein families in and 14 protein families in . Serine proteases (22.31%) and metalloproteinases (21.06%) were the dominant proteins in the venom of ; while metalloproteinases (34.84%), phospholipase As (21.19%) and serine proteases (15.50%) represent the major toxins in the venom. Other protein families such as three-finger toxins and cysteine-rich venom proteins were detected in low proportions. This study provides an insight into the venom proteomic analysis of the two Nigerian viper species, which could be useful in identifying the toxin families to be neutralized in case of envenomation.
蛇咬伤中毒仍然是一种被忽视的热带疾病，对健康构成严重危害，尤其是对非洲农村居民。在尼日利亚，蝰蛇导致的死亡人数最多。采用亲水作用液相色谱联用LC-MS/MS分析锯鳞蝰和鼓腹咝蝰的粗毒，以了解它们的毒液蛋白质组特征。所得结果显示，对粗毒进行无胶蛋白质组分析分别鉴定出85种和79种蛋白质。两种蛇共有78种蛋白质，相似性得分91.8%。鉴定出的蛋白质在锯鳞蝰毒液中属于18个蛋白家族，在鼓腹咝蝰毒液中属于14个蛋白家族。丝氨酸蛋白酶（22.31%）和金属蛋白酶（21.06%）是锯鳞蝰毒液中的主要蛋白质；而金属蛋白酶（34.84%）、磷脂酶A（21.19%）和丝氨酸蛋白酶（15.50%）是鼓腹咝蝰毒液中的主要毒素。其他蛋白家族，如三指毒素和富含半胱氨酸的毒液蛋白，检测比例较低。本研究深入了解了两种尼日利亚蝰蛇的毒液蛋白质组分析情况，这可能有助于确定中毒时需要中和的毒素家族。
