Inoue T, Yamane H, Yamamura T, Shimono M
J Dent Res. 1987 Aug;66(8):1303-9. doi: 10.1177/00220345870660080301.
Long-term repeated administration of isoproterenol (IPR) 2 mg/100 g bw, once daily for ten days, resulted in morphological changes in the intercellular junctions of rat submandibular glands, which were investigated by means of the freeze-fracture technique. A significantly increased number of tight-junctional strands was present. These junctional strands extended much deeper toward the basal membrane than those in normal acinar cells. The basal frontier strands that branched from the networks of tight junctions were elongated and had either free-endings or terminal loops, which were more frequently observed in the IPR-treated acinar cells than in untreated acinar cells. Some of the strands of tight junctions were connected to small gap junctions. The diameters of gap junctions were not significantly different from those of control acinar cells. However, smooth areas devoid of particles were found intermingling with the usual packed particles in irregularly shaped small gap junctions. There was no significant difference between the desmosomes of IPR-treated and untreated acinar cells, in terms of either morphology or distribution. These changes in junctional morphology in the IPR-treated acinar cells resemble those seen in salivary glands during development, and in some experimental conditions including tumorous changes.
每天一次，连续十天给大鼠腹腔注射2毫克/100克体重的异丙肾上腺素（IPR），长期重复给药后，采用冷冻蚀刻技术对大鼠下颌下腺细胞间连接的形态变化进行了研究。紧密连接链的数量显著增加。这些连接链比正常腺泡细胞中的连接链向基底膜延伸得更深。从紧密连接网络分支出来的基底前沿链伸长，有自由末端或末端环，在经IPR处理的腺泡细胞中比未处理的腺泡细胞中更常见。一些紧密连接链与小间隙连接相连。间隙连接的直径与对照腺泡细胞的直径无显著差异。然而，在不规则形状的小间隙连接中，发现无颗粒的光滑区域与通常堆积的颗粒混合在一起。在形态或分布方面，经IPR处理和未处理的腺泡细胞的桥粒之间没有显著差异。经IPR处理的腺泡细胞中连接形态的这些变化类似于发育过程中唾液腺以及包括肿瘤变化在内的一些实验条件下所见的变化。
