Bawden J W, Deaton T G, Crenshaw M A
J Dent Res. 1987 Aug;66(8):1360-3. doi: 10.1177/00220345870660081501.
This study investigated the diffusion of fluoride through the enamel organ in vitro. The rat molar explants used were entirely in the secretory stage or predominantly in the maturation stage of enamel formation. The removal of the enamel organ or metabolic inhibition with iodoacetate caused significant increases in enamel fluoride uptake at both stages of enamel formation. Inhibition with dinitrophenol caused a significant increase only in the maturation phase. Uptake of fluoride in enamel was related to the fluoride concentration in the medium, except in the maturation stage explants, where increasing the medium fluoride concentration from 0.05 ppm to 0.08 ppm did not significantly increase fluoride uptake at any of the three observation times. The findings indicate that the enamel organ exists as a diffusion-limiting membrane to the movement of fluoride from the extracellular fluid compartment to the developing enamel.
本研究在体外研究了氟化物通过牙釉质器的扩散情况。所用的大鼠磨牙外植体完全处于牙釉质形成的分泌期或主要处于成熟期。去除牙釉质器或用碘乙酸进行代谢抑制，在牙釉质形成的两个阶段均导致牙釉质氟摄取量显著增加。用二硝基酚抑制仅在成熟期导致显著增加。牙釉质中氟的摄取与培养基中的氟浓度有关，但在成熟期外植体中除外，在该阶段，将培养基氟浓度从0.05 ppm增加到0.08 ppm，在三个观察时间点中的任何一个时间点，氟摄取量均未显著增加。研究结果表明，牙釉质器作为一种扩散限制膜，限制了氟化物从细胞外液区室向正在发育的牙釉质的移动。
