Bawden J W, Deaton T G, Crenshaw M A
J Dent Res. 1987 Oct;66(10):1587-90. doi: 10.1177/00220345870660101501.
Eight- and 12-day-old rat pups were injected intraperitoneally with fluoride. Plasma, molar enamel, and bone samples were collected at observation times up to six hr after injection. In a second series, adult rats maintained for six weeks on water containing 5 ppm F were injected with fluoride. Plasma, incisor enamel, and bone samples were collected at the same observation times as those used in the first series. Fluoride assays were conducted by means of the microdiffusion, ion-selective-electrode method. In the suckling rats, plasma [F] levels peaked at 15 min and returned nearly to baseline in one hr. Significant increases in the [F] of developing enamel and bone were observed. No significant decline from the peak [F] seen in the hard tissues was observed over the six-hour period. Similar results were seen in the developing enamel of the adult rats. The data gave no evidence of a short-term reversible component of fluoride uptake in developing enamel. Apparent increases in F uptake in enamel and bone beyond peak plasma values suggest the presence of a diffusion-limiting membrane for fluoride from the extracellular fluids into the mineralizing matrix.
给8日龄和12日龄的幼鼠腹腔注射氟化物。在注射后长达6小时的观察时间采集血浆、磨牙牙釉质和骨骼样本。在第二个实验系列中，给饮用含5 ppm氟的水达六周的成年大鼠注射氟化物。在与第一个系列相同的观察时间采集血浆、切牙牙釉质和骨骼样本。采用微扩散离子选择电极法进行氟化物测定。在乳鼠中，血浆氟水平在15分钟时达到峰值，并在1小时内几乎恢复到基线水平。观察到发育中的牙釉质和骨骼中的氟显著增加。在6小时期间，硬组织中观察到的氟峰值未见显著下降。在成年大鼠发育中的牙釉质中也观察到类似结果。数据没有提供发育中牙釉质摄取氟存在短期可逆成分的证据。牙釉质和骨骼中氟摄取量明显高于血浆峰值，这表明存在一个限制氟从细胞外液扩散到矿化基质的膜。
