Department of Medical Biology, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey.
Department of Biophysics, Faculty of Medicine, Istanbul Aydin University, Istanbul, Turkey.
Biol Trace Elem Res. 2022 Feb;200(2):535-542. doi: 10.1007/s12011-021-03002-8. Epub 2021 Nov 12.
The link between chronic gastritis and chemosensory receptors is considered promising for disease prediction and treatment. The transient receptor potential melastatin member channel 5 (TRPM5) is an ion channel and may be a chemosensor in the gastrointestinal tract. Trace elements and minerals involved in many protein structures can change ion channel activity. Our study aimed to determine trace element and mineral levels according to TRPM5 Asn235Ser missense polymorphism distributions in patients with H. pylori (+) and (-) gastritis and to uncover their possible association with disease pathogenesis. In 109 volunteer patients diagnosed with gastritis [64 H. pylori (+), 45 H. pylori (-)], TRPM5 (Asn235Ser) polymorphism was detected by Kompetitive Allele-Specific PCR method (KASP), but no difference was found between the groups. There are differences between the serum trace element (Se, Zn, Cu, Mg, and Cu/Zn) levels of the two groups. High serum Se and Cu/Zn ratios were detected in H. pylori (+) patients. Cu/Zn ratio can be used as a marker of bacterial inflammation. The amount of Se was found to be statistically significant in the serums of H. pylori (+) patients carrying TT and CT genotype, while the amounts of Zn, Cu, and Mg were found as significant of H. pylori (-) patients. The relationship of Se with the H. pylori needs to be investigated further. In addition, H. pylori (+) patients have a significantly higher neutrophils/lymphocytes ratio (NLR) compared to those with H. pylori (-) NLR can be used as an indicator of inflammation.
慢性萎缩性胃炎与化学感受器受体之间的联系被认为对疾病预测和治疗具有重要意义。瞬时受体电位 melastatin 成员通道 5（TRPM5）是一种离子通道，可能是胃肠道中的化学感受器。参与许多蛋白质结构的微量元素和矿物质可以改变离子通道活性。我们的研究旨在根据幽门螺杆菌（+）和（-）胃炎患者 TRPM5 Asn235Ser 错义突变的分布情况，确定微量元素和矿物质水平，并揭示其与疾病发病机制的可能关联。在 109 名诊断为胃炎的志愿者患者（64 名幽门螺杆菌（+），45 名幽门螺杆菌（-））中，通过 Kompetitive Allele-Specific PCR 方法（KASP）检测 TRPM5（Asn235Ser）多态性，但两组之间无差异。两组血清微量元素（硒、锌、铜、镁和铜/锌）水平存在差异。幽门螺杆菌（+）患者血清中硒和铜/锌比值较高。铜/锌比值可作为细菌炎症的标志物。在携带 TT 和 CT 基因型的幽门螺杆菌（+）患者血清中发现硒的含量具有统计学意义，而在幽门螺杆菌（-）患者中发现锌、铜和镁的含量具有统计学意义。需要进一步研究硒与幽门螺杆菌的关系。此外，幽门螺杆菌（+）患者的中性粒细胞/淋巴细胞比值（NLR）明显高于幽门螺杆菌（-）患者。NLR 可作为炎症的指标。
