pH 值和水力停留时间调节在厌氧发酵中的作用：重点关注挥发性脂肪酸的产生/分布、微生物群落演替和相互关联。
pH and hydraulic retention time regulation for anaerobic fermentation: Focus on volatile fatty acids production/distribution, microbial community succession and interactive correlation.
机构信息
Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China; University of Chinese Academy of Sciences, Beijing 100049, China; School of Environment and Nature Resources, Renmin University of China, Beijing 100872, China; Key Laboratory of Energy Resource Utilization from Agriculture Residue, Ministry of Agriculture and Rural Affairs, China.
Key Laboratory of Urban Pollutant Conversion, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, China.
出版信息
Bioresour Technol. 2022 Mar;347:126310. doi: 10.1016/j.biortech.2021.126310. Epub 2021 Nov 9.
Enriching suitable fermentative products by optimizing operation conditions could effectively improve the efficiency of anaerobic digestion. In the present study, pH (5.0-6.0) and hydraulic retention time (HRT) (2 h-12 h) were regulated for volatile fatty acids (VFAs) production during glucose fermentation in acidogenic continuous stirred tank reactor (CSTR). Results showed that acetate and butyrate dominated during pH regulation. HRT reduction favored butyrate production and formate retainment. Maximum total VFAs production with highest acetate content was achieved at pH of 6.0 and HRT of 6 h. Microbial analysis revealed that Clostridium_sensu_stricto_1 was predominant butyrate producer during pH regulation, and Bacteroides was main contributor when HRT shorter than 6 h. In addition to acetyl-CoA pathway, acetate could also be produced via homoacetogenesis by Parabacteroides, UCG-004 and norank_f__Acidaminococcaceae. These results would give guidance for enhancing targeted VFAs products by optimizing operational parameters or bio-augmentation with specific bacteria.
通过优化操作条件来丰富合适的发酵产物，可以有效地提高厌氧消化的效率。在本研究中，通过调节 pH 值（5.0-6.0）和水力停留时间（HRT）（2 h-12 h），在产酸连续搅拌罐反应器（CSTR）中葡萄糖发酵过程中生产挥发性脂肪酸（VFAs）。结果表明，在 pH 值调节过程中，乙酸和丁酸占主导地位。HRT 缩短有利于丁酸的产生和甲酸的保留。在 pH 值为 6.0 和 HRT 为 6 h 时，可获得最大的总 VFAs 产量和最高的乙酸含量。微生物分析表明，Clostridium_sensu_stricto_1 是 pH 值调节过程中主要的丁酸产生菌，而当 HRT 短于 6 h 时，Bacteroides 是主要贡献者。除乙酰辅酶 A 途径外，Parabacteroides、UCG-004 和 norank_f__Acidaminococcaceae 还可以通过同型乙酰辅酶 A 生成途径产生乙酸。这些结果将为通过优化操作参数或通过特定细菌的生物增强来增强目标 VFAs 产物提供指导。
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