UBMD Department of Orthopaedics and Sports Medicine, Jacobs School of Medicine and Biomedical Sciences, State University of New York at Buffalo, Buffalo, New York, U.S.A..
Department of Orthopaedic Surgery, UConn Health, Farmington, Connecticut, U.S.A.
Arthroscopy. 2022 Apr;38(4):1115-1123. doi: 10.1016/j.arthro.2021.10.029. Epub 2021 Nov 10.
To build upon previous literature to identify a complete analysis of cellular contents of subacromial bursal tissue as well as the matrix surrounding the rotator cuff.
Samples of subacromial bursal tissue and surrounding matrix milieu from above the rotator cuff tendon and above the rotator cuff muscle bellies were obtained from 10 patients undergoing arthroscopic rotator cuff repair. Samples were analyzed using fluorescent-activated cell sorting and histologic analysis with staining protocols (Oil Red O, Alcian Blue, and Picro-Sirius Red), for identification of matrix components, including fat, proteoglycans, and collagen.
Progenitor cells and fibroblast-type cells were present in significant amounts in subacromial bursal tissue in both tissues obtained from over the tendinous and muscle belly portions. Markers for neural tissue, myeloid cells, and megakaryocytes also were present to a lesser extent. There were prominent amounts of fat and proteoglycans present in the matrix, based on ImageJ analysis of stained histologic slides.
The subacromial bursal tissue and surrounding matrix of patients undergoing rotator cuff repair contains progenitor cells in significant concentrations both over the tendon and muscle belly of the rotator cuff.
This presence of progenitor cells, in particular, in the subacromial bursal tissue provides a potential basis for future applications of augmentation purposes in rotator cuff healing, and calls into question the practice of routine bursectomy. As the potential role of bursal tissue contents in growth and regeneration in the setting of rotator cuff healing is more well understood, maintaining this tissue may become more relevant. Concentration of these cellular components for use in autologous re-implantation is also an avenue of interest.
在前人文献的基础上，对肩峰下滑囊组织的细胞内容物以及肩袖周围基质进行全面分析。
从 10 例接受关节镜肩袖修复术的患者的肩袖肌腱上方和肩袖肌肉上方获取肩峰下滑囊组织和周围基质样本。使用荧光激活细胞分选和组织学分析（油红 O、阿利新蓝和苦味酸天狼猩红染色）对样本进行分析，以鉴定基质成分，包括脂肪、蛋白聚糖和胶原。
在取自肌腱上方和肌肉上方两部分的肩峰下滑囊中，均存在大量的祖细胞和成纤维细胞样细胞。神经组织、髓样细胞和巨核细胞的标志物也存在，但数量较少。根据染色组织学切片的 ImageJ 分析，基质中存在大量的脂肪和蛋白聚糖。
接受肩袖修复术的患者的肩峰下滑囊组织和周围基质中，无论是在肩袖肌腱上方还是肌肉上方，均存在大量的祖细胞。
特别是在肩峰下滑囊组织中存在祖细胞，为肩袖愈合中增强目的的未来应用提供了潜在基础，并对常规滑囊切除术的做法提出了质疑。随着对肩峰下滑囊组织内容物在肩袖愈合中的生长和再生潜在作用的理解更加深入，保留这种组织可能变得更加重要。浓缩这些细胞成分以供自体再植入的应用也是一个研究方向。
