Schlicker E, Fink K, Göthert M
Naunyn Schmiedebergs Arch Pharmacol. 1987 Jun;335(6):646-51. doi: 10.1007/BF00166981.
Superfused rat brain cortex slices, hypothalamic slices and cortex synaptosomes preincubated with 3H-serotonin or 3H-noradrenaline were used to study the effects of eicosanoids on tritium overflow evoked either electrically (3 Hz; slices) or by potassium 12 mmol/l (synaptosomes). 1. The electrically evoked 3H overflow from cortex slices preincubated with 3H-serotonin was inhibited by prostaglandins E1 and E2 and by the prostacyclin analogue iloprost. No effect was seen with prostaglandin F2 alpha, prostaglandin D2, CG 4203 (another prostacyclin analogue), U 46619 (a thromboxane A2 analogue) and leukotriene C4. The same held true for indomethacin and the prostaglandin receptor antagonists SC 19220 and N-0164. The inhibitory effect of prostaglandin E2 was slightly more pronounced in the presence of indomethacin than in its absence, but was not affected by SC 19220, N-0164 or forskolin plus AH 21-132 (an inhibitor of cAMP phosphodiesterase). Yohimbine and the serotonin receptor antagonist metitepin failed to influence the inhibitory effect of prostaglandin E1. 2. The potassium-evoked 3H overflow from cortex synaptosomes preincubated with 3H-serotonin was inhibited by prostaglandin E2. 3. Prostaglandin E2 also inhibited the electrically evoked 3H overflow from hypothalamic slices preincubated with 3H-serotonin. 4. The electrically evoked 3H overflow from cortex slices preincubated with 3H-noradrenaline was inhibited by prostaglandin E2, but was not affected by SC 19220, which, in turn, did also not alter the effect of prostaglandin E2. The present results are compatible with the view that presynaptic SC 19220-insensitive prostaglandin E receptors may be involved in the inhibitory effect of prostaglandins E1 and E2 on serotonin (and noradrenaline) release.
使用预先用³H - 5 - 羟色胺或³H - 去甲肾上腺素孵育的大鼠脑皮质切片、下丘脑切片和皮质突触体，研究类花生酸对电刺激（3Hz；切片）或12mmol/L钾离子刺激（突触体）诱发的氚溢出的影响。1. 预先用³H - 5 - 羟色胺孵育的皮质切片，其电诱发的³H溢出受到前列腺素E1和E2以及前列环素类似物伊洛前列素的抑制。前列腺素F2α、前列腺素D2、CG 4203（另一种前列环素类似物）、U 46619（一种血栓素A2类似物）和白三烯C4未见作用。吲哚美辛以及前列腺素受体拮抗剂SC 19220和N - 0164也有同样的情况。在存在吲哚美辛时，前列腺素E2的抑制作用比不存在时略为明显，但不受SC 19220、N - 0164或福斯可林加AH 21 - 132（一种环磷酸腺苷磷酸二酯酶抑制剂）的影响。育亨宾和5 - 羟色胺受体拮抗剂美替平未能影响前列腺素E1的抑制作用。2. 预先用³H - 5 - 羟色胺孵育的皮质突触体，其钾离子诱发的³H溢出受到前列腺素E2的抑制。3. 前列腺素E2也抑制预先用³H - 5 - 羟色胺孵育的下丘脑切片的电诱发³H溢出。4. 预先用³H - 去甲肾上腺素孵育的皮质切片，其电诱发的³H溢出受到前列腺素E2的抑制，但不受SC 19220的影响，反过来，SC 19220也不改变前列腺素E2的作用。目前的结果与以下观点一致，即突触前对SC 19220不敏感的前列腺素E受体可能参与前列腺素E1和E2对5 - 羟色胺（和去甲肾上腺素）释放的抑制作用。
