Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
Int J Mol Sci. 2021 Oct 21;22(21):11367. doi: 10.3390/ijms222111367.
MicroRNAs (miRNAs) handle immune response to pathogens by adjusting the function of target genes in plants. However, the experimentally documented miRNA/target modules implicated in the interplay between rice and pv. () are still in the early stages. Herein, the expression of osa-miR1432 was induced in resistant genotype IRBB5, but not susceptible genotype IR24, under strain PXO86 attack. Overexpressed osa-miR1432 heightened rice disease resistance to , indicated by enhancive enrichment of defense marker genes, raised reactive oxygen species (ROS) levels, repressed bacterial growth and shortened leaf lesion length, whilst the disruptive accumulation of osa-miR1432 accelerated rice susceptibility to infection. Noticeably, () was experimentally confirmed as a target gene of osa-miR1432, and the overexpressing transgenic plants exhibited compromised resistance to infestation. Our results indicate that osa-miR1432 and were differently responsive to invasion at the transcriptional level and fine-tune rice resistance to infection, which may be referable in resistance gene discovery and valuable in the pursuit of improving resistance in rice breeding.
MicroRNAs (miRNAs) 通过调节植物靶基因的功能来控制对病原体的免疫反应。然而，在水稻与 pv. ()互作中涉及的经实验证实的 miRNA/靶模块仍处于早期阶段。在此，在菌株 PXO86 攻击下，抗性基因型 IRBB5 中诱导表达了 osa-miR1432，但敏感基因型 IR24 中没有诱导表达。过表达的 osa-miR1432 增强了水稻对 的抗病性，表现为防御标记基因的富集增强、活性氧 (ROS) 水平升高、抑制细菌生长和缩短叶片病斑长度，而 osa-miR1432 的破坏积累加速了水稻对 感染的敏感性。值得注意的是，()被实验证实是 osa-miR1432 的靶基因，过表达 的转基因植物对 侵染的抗性受损。我们的结果表明，osa-miR1432 和 在转录水平上对 入侵的反应不同，精细调控了水稻对 感染的抗性，这可能有助于抗病基因的发现，并在提高水稻抗性的育种中具有价值。
