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OsamiR11117 靶向调控水稻对白粉病的免疫

Osa-miR11117 Targets to Regulate Rice Immunity against the Blast Fungus .

机构信息

National Plant Space Breeding Engineering Technology Research Center, Guangdong Key Laboratory of Plant Molecular Breeding, South China Agricultural University, Guangzhou 510642, China.

出版信息

Int J Mol Sci. 2023 Nov 7;24(22):16052. doi: 10.3390/ijms242216052.

Abstract

The intricate regulatory process governing rice immunity against the blast fungus remains a central focus in plant-pathogen interactions. In this study, we investigated the important role of Osa-miR11117, an intergenic microRNA, in regulating rice defense mechanisms. Stem-loop qRT-PCR analysis showed that Osa-miR11117 is responsive to infection, and overexpression of Osa-miR11117 compromises blast resistance. Green fluorescent protein (GFP)-based reporter assay indicated is one direct target of Osa-miR11117. Furthermore, qRT-PCR analysis showed that reacts to infection and polyamine (PA) treatment. In addition, regulates rice resistance to through the regulation of PA accumulation and the expression of the ethylene (ETH) signaling genes. Taken together, these results suggest that Osa-miR11117 is targeting to regulate blast resistance by adjusting PA metabolism and ETH signaling pathways.

摘要

调控水稻对稻瘟病菌免疫的复杂调控过程仍然是植物-病原菌相互作用的研究重点。在本研究中,我们研究了非编码 microRNAosa-miR11117 在调控水稻防御机制中的重要作用。茎环 qRT-PCR 分析表明,osa-miR11117 对稻瘟病菌的侵染有响应,而过表达 osa-miR11117 会削弱稻瘟病抗性。基于绿色荧光蛋白(GFP)的报告基因检测表明,是 osa-miR11117 的一个直接靶标。此外,qRT-PCR 分析表明,在稻瘟病菌侵染和多胺(PA)处理时表达。另外,通过调节 PA 积累和乙烯(ETH)信号基因的表达来调控水稻对稻瘟病菌的抗性。综上所述,这些结果表明,osa-miR11117 通过调节 PA 代谢和 ETH 信号通路,靶向 来调节稻瘟病抗性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8d06/10670930/872f430f06db/ijms-24-16052-g001.jpg

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