Mastrevirus Rep 和 RepA 蛋白抑制从头转录基因沉默。

Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing.

机构信息

Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, 5-1-5, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan.

出版信息

Int J Mol Sci. 2021 Oct 24;22(21):11462. doi: 10.3390/ijms222111462.

DOI:10.3390/ijms222111462

PMID:34768892

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8584122/

Abstract

Transcriptional gene silencing (TGS) in plants is a defense mechanism against DNA virus infection. The genomes of viruses in the family encode several TGS suppressors. In this study, we induced de novo TGS against the transgenic gene encoding green fluorescent protein by expressing a hairpin-shaped self-complementary RNA corresponding to the enhancer region of the 35S promoter (hpE35S). In addition, we examined the TGS suppression activity of proteins encoded in the genome of (TYDV, genus ). The results show that the replication-associated protein (Rep) and RepA encoded by TYDV have TGS suppressor activity and lead to decreased accumulation of 24-nt siRNAs. These results suggest that Rep and RepA can block the steps before the loading of siRNAs into Argonaute (AGO) proteins. This is the first report of TGS suppressors in the genus .

摘要

植物中的转录基因沉默 (TGS) 是一种针对 DNA 病毒感染的防御机制。家族病毒的基因组编码了几种 TGS 抑制剂。在这项研究中，我们通过表达与 35S 启动子增强子区域对应的发夹状自互补 RNA 诱导针对转基因为绿色荧光蛋白的基因的从头 TGS（hpE35S）。此外，我们还研究了（TYDV，属）基因组中编码的蛋白质的 TGS 抑制活性。结果表明，TYDV 编码的复制相关蛋白 (Rep) 和 RepA 具有 TGS 抑制活性，导致 24-nt siRNA 的积累减少。这些结果表明 Rep 和 RepA 可以阻断 siRNA 加载到 Argonaute (AGO) 蛋白之前的步骤。这是属中 TGS 抑制剂的首次报道。