Department of Microbiology and Biotechnology, Faculty of Biotechnology and Animal Husbandry, West Pomeranian University of Technology in Szczecin, Piastów 45, 70-311 Szczecin, Poland.
Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Medical University of Wroclaw, Borowska 211a, 50-534 Wrocław, Poland.
Int J Mol Sci. 2021 Oct 26;22(21):11551. doi: 10.3390/ijms222111551.
Methicillin-resistant strains of (MRSA) have become a global issue for healthcare systems due to their resistance to most β-lactam antibiotics, frequently accompanied by resistance to other classes of antibiotics. In this work, we analyzed the impact of combined use of rotating magnetic field (RMF) with various classes of antibiotics (β-lactams, glycopeptides, macrolides, lincosamides, aminoglycosides, tetracyclines, and fluoroquinolones) against nine strains (eight methicillin-resistant and one methicillin-sensitive). The results indicated that the application of RMF combined with antibiotics interfering with cell walls (particularly with the β-lactam antibiotics) translate into favorable changes in staphylococcal growth inhibition zones or in minimal inhibitory concentration values compared to the control settings, which were unexposed to RMF. As an example, the MIC value of cefoxitin was reduced in all MRSA strains by up to 42 times. Apart from the β-lactams, the reduced MIC values were also found for erythromycin, clindamycin, and tetracycline (three strains), ciprofloxacin (one strain), gentamicin (six strains), and teicoplanin (seven strains). The results obtained with the use of in vitro biofilm model confirm that the disturbances caused by RMF in the bacterial cell walls increase the effectiveness of the antibiotics towards MRSA. Because the clinical demand for new therapeutic options effective against MRSA is undisputable, the outcomes and conclusions drawn from the present study may be considered an important road into the application of magnetic fields to fight infections caused by methicillin-resistant staphylococci.
耐甲氧西林金黄色葡萄球菌（MRSA）由于对大多数β-内酰胺类抗生素具有耐药性，并且经常伴有对其他类别的抗生素的耐药性，因此已成为医疗保健系统的全球性问题。在这项工作中，我们分析了旋转磁场（RMF）与各种类别的抗生素（β-内酰胺类，糖肽类，大环内酯类，林可酰胺类，氨基糖苷类，四环素类和氟喹诺酮类）联合使用对九株金黄色葡萄球菌（八株耐甲氧西林金黄色葡萄球菌和一株耐甲氧西林敏感菌）的影响。结果表明，RMF 与干扰细胞壁的抗生素（特别是与β-内酰胺类抗生素）联合应用，与未暴露于 RMF 的对照相比，金黄色葡萄球菌生长抑制区或最小抑菌浓度值发生了有利变化。例如，所有耐甲氧西林金黄色葡萄球菌菌株的头孢西丁 MIC 值降低了 42 倍。除了β-内酰胺类抗生素外，红霉素、克林霉素和四环素（三株）、环丙沙星（一株）、庆大霉素（六株）和替考拉宁（七株）的 MIC 值也降低了。使用体外生物膜模型获得的结果证实，RMF 对细菌细胞壁造成的干扰增加了抗生素对耐甲氧西林金黄色葡萄球菌的有效性。由于对有效对抗耐甲氧西林金黄色葡萄球菌的新治疗方法的临床需求是不可争议的，因此本研究的结果和结论可以被认为是将磁场应用于治疗耐甲氧西林金黄色葡萄球菌感染的重要途径。
