卵巢癌患者 PD-L1/PD-L2 表达的抗原呈递细胞的临床和预后价值。

Clinical and Prognostic Value of Antigen-Presenting Cells with PD-L1/PD-L2 Expression in Ovarian Cancer Patients.

机构信息

Independent Laboratory of Cancer Diagnostics and Immunology, I Chair and Department of Oncological Gynaecology and Gynaecology, Medical University of Lublin, 20-093 Lublin, Poland.

I Chair and Department of Oncological Gynaecology and Gynaecology, Medical University of Lublin, 20-081 Lublin, Poland.

出版信息

Int J Mol Sci. 2021 Oct 26;22(21):11563. doi: 10.3390/ijms222111563.

The latest literature demonstrates the predominant role of the programmed cell death axis (PD-1/PD-L1/PD-L2) in ovarian cancer (OC) pathogenesis. However, data concerning this issue is ambiguous. Our research aimed to evaluate the clinical importance of PD-L1/PD-L2 expression in OC environments. We evaluated the role of PD-L1/PD-L2 in OC patients ( = 53). The analysis was performed via flow cytometry on myeloid (mDCs) and plasmacytoid dendritic cells (pDCs) and monocytes/macrophages (MO/MA) in peripheral blood, peritoneal fluid (PF), and tumor tissue (TT). The data were correlated with clinicopathological characteristics and prognosis of OC patients. The concentration of soluble PD-L1 (sPD-L1) and PD-1 in the plasma and PF were determined by ELISA. We established an accumulation of PD-L1/PD-L2 mDCs, pDCs, and MA in the tumor microenvironment. We showed an elevated level of sPD-L1 in the PF of OC patients in comparison to plasma and healthy subjects. sPD-L1 levels in PF showed a positive relationship with Ca125 concentration. Moreover, we established an association between higher sPD-L1 levels in PF and shorter survival of OC patients. An accumulation of PD-L1/PD-L2 mDCs, pDCs, and MA in the TT and high sPD-L1 levels in PF could represent the hallmark of immune regulation in OC patients.

最新文献表明，程序性死亡受体 1/配体 1/配体 2（PD-1/PD-L1/PD-L2）轴在卵巢癌（OC）发病机制中起主要作用。然而，关于这个问题的数据是模糊的。我们的研究旨在评估 PD-L1/PD-L2 在 OC 环境中的临床重要性。我们评估了 PD-L1/PD-L2 在 OC 患者（=53）中的作用。通过流式细胞术在外周血、腹腔液（PF）和肿瘤组织（TT）中的髓样树突状细胞（mDC）和浆细胞样树突状细胞（pDC）和单核细胞/巨噬细胞（MO/MA）上进行分析。将数据与 OC 患者的临床病理特征和预后相关联。通过 ELISA 测定血浆和 PF 中可溶性 PD-L1（sPD-L1）和 PD-1 的浓度。我们在肿瘤微环境中建立了 PD-L1/PD-L2 mDC、pDC 和 MA 的积累。我们发现 OC 患者 PF 中的 sPD-L1 水平高于血浆和健康受试者。PF 中的 sPD-L1 水平与 Ca125 浓度呈正相关。此外，我们还发现 PF 中较高的 sPD-L1 水平与 OC 患者的生存时间较短有关。TT 中 PD-L1/PD-L2 mDC、pDC 和 MA 的积累和 PF 中高 sPD-L1 水平可能代表 OC 患者免疫调节的特征。