Department of Regenerative Medicine, Yokohama City University Graduate School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
Division of Regenerative Medicine, Center for Stem Cell Biology and Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan.
Int J Mol Sci. 2021 Oct 27;22(21):11589. doi: 10.3390/ijms222111589.
Liver transplantation is the most effective treatment for end-stage cirrhosis. However, due to serious donor shortages, new treatments to replace liver transplantation are sorely needed. Recent studies have focused on novel therapeutic methods using hepatocytes and induced pluripotent stem cells, we try hard to develop methods for transplanting these cells to the liver surface. In the present study, we evaluated several methods for their efficiency in the detachment of serous membrane covering the liver surface for transplantation to the liver surface. The liver surface of dipeptidyl peptidase IV (DPPIV)-deficient rats in a cirrhosis model was detached by various methods, and then fetal livers from DPPIV-positive rats were transplanted. We found that the engraftment rate and area as well as the liver function were improved in rats undergoing transplantation following serous membrane detachment with an ultrasonic homogenizer, which mimics the Cavitron Ultrasonic Surgical Aspirator (CUSA), compared with no detachment. Furthermore, the bleeding amount was lower with the ultrasonic homogenizer method than with the needle and electric scalpel methods. These findings provide evidence that transplantation to the liver surface with serous membrane detachment using CUSA might contribute to the development of new treatments for cirrhosis using cells or tissues.
肝移植是治疗终末期肝硬化最有效的方法。然而，由于严重的供体短缺，迫切需要新的治疗方法来替代肝移植。最近的研究集中在使用肝细胞和诱导多能干细胞的新型治疗方法上，我们努力开发将这些细胞移植到肝表面的方法。在本研究中，我们评估了几种用于分离覆盖肝表面的浆膜以移植到肝表面的方法的效率。通过各种方法分离二肽基肽酶 IV（DPPIV）缺陷型大鼠肝硬化模型中的肝表面，然后移植 DPPIV 阳性大鼠的胎肝。我们发现，与未分离相比，使用模拟 Cavitron Ultrasonic Surgical Aspirator（CUSA）的超声匀浆器进行浆膜分离后，移植大鼠的植入率、面积和肝功能均得到改善。此外，与针和电刀方法相比，超声匀浆器法的出血量更低。这些发现为使用细胞或组织进行肝表面移植结合浆膜分离的 CUSA 提供了证据，可能有助于开发治疗肝硬化的新方法。
