Department of Physical and Quantum Chemistry, Faculty of Chemistry, Wroclaw University of Science and Technology, Wybrzeze Wyspianskiego 27, 50-370 Wroclaw, Poland.
Int J Mol Sci. 2021 Oct 29;22(21):11776. doi: 10.3390/ijms222111776.
Amphoteric amphiphilic compounds, due to their unique properties, may represent a group of safe and biocompatible surface-active agents for effective colloidal stabilization of nanoformulations. For this reason, the aim of this work was to develop and characterize the oil-in-water nanoemulsions based on two betaine-derived surfactants with high biodegradability, i.e., cocamidopropyl betaine and coco-betaine. In the first step, we investigated ternary phase diagrams of surfactant-oil-water systems containing different weight ratios of surfactant and oil, as the betaine-type surfactant entity (S), linoleic acid, or oleic acid as the oil phase (O), and the aqueous phase (W) using the titration-ultrasound approach. All the received nanoemulsion systems were then characterized upon droplets size (dynamic light scattering), surface charge (electrophoretic light scattering), and morphology (transmission electron as well as atomic force microscopy). Thermal and spinning tests revealed the most stable compositions, which were subjected to further kinetic stability analysis, including turbidimetric evaluation. Finally, the backscattering profiles revealed the most promising candidate with a size <200 nm for potential delivery of active agents in the future cosmetic, pharmaceutical, and biomedical applications.
两性两亲化合物由于其独特的性质，可能代表了一组安全且生物相容的表面活性剂，可有效胶态稳定纳米制剂。基于此，本工作旨在开发并表征基于两种具有高生物降解性的甜菜碱型表面活性剂（即椰油酰胺丙基甜菜碱和椰油基甜菜碱）的油包水纳米乳。在第一步中，我们使用滴定-超声法研究了含有不同表面活性剂和油重量比的表面活性剂-油-水三元相图，其中甜菜碱型表面活性剂实体（S）为亚油酸或油酸作为油相（O），水相（W）。然后对所有得到的纳米乳体系进行了液滴尺寸（动态光散射）、表面电荷（电泳光散射）和形态（透射电子显微镜和原子力显微镜）的表征。热和纺丝试验揭示了最稳定的组成，对其进行了进一步的动力学稳定性分析，包括浊度评估。最后，反向散射谱揭示了最有前途的候选者，其尺寸<200nm，有望在未来的化妆品、制药和生物医学应用中输送活性药物。
