Department of Environmental Sciences, Faculty of Life & Environmental Sciences, University of Yamanashi, Takeda, Kofu, Yamanashi 400-8510, Japan.
Aridland Research Center, Tottori University, Hamasaka, Tottori 680-8550, Japan.
Int J Mol Sci. 2021 Oct 30;22(21):11813. doi: 10.3390/ijms222111813.
L. (common ice plant) is an edible halophyte. However, if ice plants are used to phytoremediate salinity soil, there are problems of slow initial growth, and a long period before active NaCl uptake occurs under higher salinity conditions. Application of endophytic bacteria may improve the problem, but there remain gaps in our understanding of how endophytic bacteria affect the growth and the biochemical and physiological characteristics of ice plants. The aims of this study were to identify growth-promoting endophytic bacteria from the roots of ice plants and to document the metabolomic response of ice plants after application of selected endophytic bacteria. Two plant growth-promoting endophytic bacteria were selected on the basis of their ability to promote ice plant growth. The two strains putatively identified as spp. and spp. significantly promoted ice plant growth, at 2-times and 2.5-times, respectively, compared with the control and also affected the metabolome of ice plants. The strain of spp. resulted in increased contents of metabolites related to the tricarboxylic acid cycle and photosynthesis. The effects of salt stress were alleviated in ice plants inoculated with the endobacterial strains, compared with uninoculated plants. A deeper understanding of the complex interplay among plant metabolites will be useful for developing microbe-assisted soil phytoremediation strategies, using species.
冰叶日中花（L.）是一种可食用的盐生植物。然而，如果冰叶日中花被用于盐土的植物修复，在高盐条件下，其初始生长缓慢，并且在开始主动吸收 NaCl 之前需要很长时间。应用内生细菌可能会改善这个问题，但我们对内生细菌如何影响冰叶日中花的生长以及其生化和生理特性的理解仍存在空白。本研究的目的是从冰叶日中花的根部中鉴定出具有促生长能力的内生细菌，并记录在应用选定的内生细菌后冰叶日中花的代谢组响应。根据促进冰叶日中花生长的能力，选择了两种植物促生内生细菌。这两株被推测为 spp. 和 spp. 的菌株分别比对照显著促进了冰叶日中花生长 2 倍和 2.5 倍，同时还影响了冰叶日中花的代谢组。 spp. 菌株导致与三羧酸循环和光合作用相关的代谢物含量增加。与未接种的植物相比，接种内生细菌的冰叶日中花减轻了盐胁迫的影响。更深入地了解植物代谢物之间的复杂相互作用将有助于开发使用 物种的微生物辅助土壤植物修复策略。
