College of Horticulture, Fujian Agriculture and Forestry University, Cangshan District, Fuzhou 350002, China.
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Jinan District, Fuzhou 350013, China.
Int J Mol Sci. 2021 Oct 31;22(21):11842. doi: 10.3390/ijms222111842.
Bud endodormancy is an important, complex process subject to both genetic and epigenetic control, the mechanism of which is still unclear. The endogenous hormone abscisic acid (ABA) and its signaling pathway play important roles in the endodormancy process, in which the type 2C protein phosphatases (PP2Cs) is key to the ABA signal pathway. Due to its excellent effect on endodormancy release, hydrogen cyanamide (HC) treatment is considered an effective measure to study the mechanism of endodormancy release. In this study, RNA-Seq analysis was conducted on endodormant floral buds of pear () with HC treatment, and the HC-induced PP2C gene was identified. Next, software prediction, expression tests and transient assays revealed that lncRNA -derived targets . The expression analysis showed that HC treatment upregulated the expression of and downregulated the expression of and . Moreover, HC treatment inhibited the accumulation of ABA signaling pathway-related genes and hydrogen peroxide (HO). Furthermore, overexpression of reduced the inhibitory effect of on the HO content. In summary, our study suggests that downregulation of -derived promotes HC-induced endodormancy release in pear plants through the PP2C-HO pathway.
芽休眠是一个受遗传和表观遗传控制的重要而复杂的过程，其机制尚不清楚。内源性激素脱落酸（ABA）及其信号通路在休眠过程中发挥重要作用，其中 2C 型蛋白磷酸酶（PP2Cs）是 ABA 信号通路的关键。由于氰氨化氢（HC）处理对解除休眠有很好的效果，因此被认为是研究解除休眠机制的有效措施。本研究对经 HC 处理的梨（）休眠芽进行了 RNA-Seq 分析，鉴定出 HC 诱导的 PP2C 基因。然后，通过软件预测、表达测试和瞬时测定揭示了 lncRNA 衍生的 可靶向. 表达分析表明，HC 处理上调了 的表达，下调了 的表达。此外，HC 处理抑制了与 ABA 信号通路相关基因和过氧化氢（HO）的积累。此外，过表达 减少了 对 HO 含量的抑制作用。综上所述，本研究表明，通过 PP2C-HO 途径下调 -衍生的 可促进 HC 诱导的梨植株休眠解除。
