College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China.
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Int J Mol Sci. 2019 Jul 18;20(14):3528. doi: 10.3390/ijms20143528.
Hydrogen cyanamide (HC) is an agrochemical compound that is frequently used to break bud dormancy in grapevines grown under mild winter conditions globally. The present study was carried out to provide an in-depth understanding of the molecular mechanism associated with HC releasing bud dormancy in grapevines. For this purpose, RNA-seq based transcriptomic and tandem mass tag (TMT)-based proteomic information was acquired and critically analyzed. The combined results of transcriptomic and proteomic analysis were utilized to demonstrate differential expression pattern of genes at the translational and transcriptional levels. The outcome of the proteomic analysis revealed that a total of 7135 proteins (-value ≤ 0.05; fold change ≥ 1.5) between the treatments (HC treated versus control) were identified, out of which 6224 were quantified. Among these differentially expressed proteins (DEPs), the majority of these proteins were related to heat shock, oxidoreductase activity, and energy metabolism. Metabolic, ribosomal, and hormonal signaling pathways were found to be significantly enriched at both the transcriptional and translational levels. It was illustrated that genes associated with metabolic and oxidoreductase activity were mainly involved in the regulation of bud dormancy at the transcriptomic and proteomic levels. The current work furnishes a new track to decipher the molecular mechanism of bud dormancy after HC treatment in grapes. Functional characterization of key genes and proteins will be informative in exactly pinpointing the crosstalk between transcription and translation in the release of bud dormancy after HC application.
氢氰酸（HC）是一种农用化学品化合物，常用于打破全球冬季条件温和地区生长的葡萄芽休眠。本研究旨在深入了解与 HC 打破葡萄芽休眠相关的分子机制。为此，我们进行了基于 RNA-seq 的转录组学和串联质量标签（TMT）-基于蛋白质组学信息的获取和关键分析。转录组学和蛋白质组学分析的综合结果用于证明基因在翻译和转录水平上的差异表达模式。蛋白质组学分析的结果表明，在处理（HC 处理与对照）之间共鉴定出 7135 种蛋白质（-值≤0.05；倍数变化≥1.5），其中 6224 种被定量。在这些差异表达蛋白（DEPs）中，大多数与热休克、氧化还原酶活性和能量代谢有关。在转录和翻译水平上，代谢、核糖体和激素信号通路都被发现显著富集。说明与代谢和氧化还原酶活性相关的基因主要参与了 HC 处理后芽休眠的调控。本研究为解析 HC 处理后葡萄芽休眠的分子机制提供了新的途径。关键基因和蛋白质的功能特征将有助于准确确定 HC 应用后转录和翻译之间芽休眠释放的串扰。
