Research Team for Geriatric Medicine (Vascular Medicine), Tokyo Metropolitan Institute of Gerontology, Tokyo 173-0015, Japan.
Cardiovascular Research Center, Lewis Katz School of Medicine, Temple University, Medical Education and Research Building, 3500N. Broad St., Philadelphia, PA 19140, USA.
Int J Mol Sci. 2021 Oct 31;22(21):11849. doi: 10.3390/ijms222111849.
The newly established mouse cortical-bone-derived stem cells (mCBSCs) are unique stem cells compared to mouse mesenchymal stem cells (mMSCs). The mCBSC-treated hearts after myocardial infarction have been reported to have greater improvement in myocardial structure and functions. In this study, we examined the stemness features, cell surface glycan profiles, and paracrine functions of mCBSCs compared with mMSCs. The stemness analysis revealed that the self-renewing capacity of mCBSCs was greater than mMSCs; however, the differentiation capacity of mCBSCs was limited to the chondrogenic lineage among three types of cells (adipocyte, osteoblast, chondrocyte). The cell surface glycan profiles by lectin array analysis revealed that α2-6sialic acid is expressed at very low levels on the cell surface of mCBSCs compared with that on mMSCs. In contrast, the lactosamine (Galβ1-4GlcNAc) structure, poly lactosamine- or poly -acetylglucosamine structure, and α2-3sialic acid on both - and -glycans were more highly expressed in mCBSCs. Moreover, we found that mCBSCs secrete a greater amount of TGF-β1 compared to mMSCs, and that the TGF-β1 contributed to the self-migration of mCBSCs and activation of fibroblasts. Together, these results suggest that unique characteristics in mCBSCs compared to mMSCs may lead to advanced utility of mCBSCs for cardiac and noncardiac repair.
新建立的鼠皮质骨源性干细胞（mCBSC）与鼠间充质干细胞（mMSC）相比是一种独特的干细胞。据报道，在心肌梗死后，接受 mCBSC 治疗的心脏在心肌结构和功能方面有更大的改善。在这项研究中，我们比较了 mCBSC 与 mMSC 的干性特征、细胞表面糖蛋白谱和旁分泌功能。干性分析表明，mCBSC 的自我更新能力大于 mMSC；然而，mCBSC 的分化能力仅限于三种细胞（脂肪细胞、成骨细胞和成软骨细胞）中的成软骨谱系。通过凝集素阵列分析的细胞表面糖蛋白谱表明，与 mMSC 相比，mCBSC 细胞表面的α2-6 唾液酸表达水平非常低。相比之下，mCBSC 中 - 和 - 聚糖上的乳糖胺（Galβ1-4GlcNAc）结构、多乳糖胺或多乙酰葡萄糖胺结构以及α2-3 唾液酸表达水平更高。此外，我们发现 mCBSC 分泌的 TGF-β1 比 mMSC 多，并且 TGF-β1 有助于 mCBSC 的自我迁移和成纤维细胞的激活。综上所述，与 mMSC 相比，mCBSC 的独特特征可能会导致 mCBSC 在心脏和非心脏修复方面的应用更加先进。
