Department of Plant Protection Biology, Swedish University of Agricultural Sciences, SE-234 22 Lomma, Sweden.
Plant Chemetics Laboratory, Department of Plant Sciences, University of Oxford, South Parks Road, Oxford OX1 3RB, UK.
Int J Mol Sci. 2021 Nov 6;22(21):12033. doi: 10.3390/ijms222112033.
Multiple biotic and abiotic stresses challenge plants growing in agricultural fields. Most molecular studies have aimed to understand plant responses to challenges under controlled conditions. However, studies on field-grown plants are scarce, limiting application of the findings in agricultural conditions. In this study, we investigated the composition of apoplastic proteomes of potato cultivar Bintje grown under field conditions, i.e., two field sites in June-August across two years and fungicide treated and untreated, using quantitative proteomics, as well as its activity using activity-based protein profiling (ABPP). Samples were clustered and some proteins showed significant intensity and activity differences, based on their field site and sampling time (June-August), indicating differential regulation of certain proteins in response to environmental or developmental factors. Peroxidases, class II chitinases, pectinesterases, and osmotins were among the proteins more abundant later in the growing season (July-August) as compared to early in the season (June). We did not detect significant differences between fungicide Shirlan treated and untreated field samples in two growing seasons. Using ABPP, we showed differential activity of serine hydrolases and β-glycosidases under greenhouse and field conditions and across a growing season. Furthermore, the activity of serine hydrolases and β-glycosidases, including proteins related to biotic stress tolerance, decreased as the season progressed. The generated proteomics data would facilitate further studies aiming at understanding mechanisms of molecular plant physiology in agricultural fields and help applying effective strategies to mitigate biotic and abiotic stresses.
多种生物和非生物胁迫挑战着生长在农田中的植物。大多数分子研究旨在了解植物在受控条件下对挑战的反应。然而，对田间生长植物的研究很少，限制了研究结果在农业条件下的应用。在这项研究中，我们使用定量蛋白质组学研究了田间条件下（即两年间的 6 月至 8 月的两个田间地点）和杀菌剂处理和未处理的土豆品种 Bintje 的质外体蛋白质组的组成，以及使用基于活性的蛋白质谱（ABPP）对其活性进行了研究。根据其田间位置和采样时间（6 月至 8 月）对样品进行聚类，一些蛋白质的强度和活性存在显著差异，表明某些蛋白质对环境或发育因素的反应存在差异调节。过氧化物酶、II 类几丁质酶、果胶酯酶和渗出素是在生长季节后期（7 月至 8 月）比早期（6 月）更为丰富的蛋白质之一。在两个生长季节中，我们未检测到杀菌剂 Shirlan 处理和未处理的田间样本之间存在显著差异。使用 ABPP，我们显示了在温室和田间条件下以及整个生长季节中丝氨酸水解酶和β-糖苷酶的活性存在差异。此外，随着季节的推移，丝氨酸水解酶和β-糖苷酶的活性，包括与生物胁迫耐受性相关的蛋白质，都有所下降。生成的蛋白质组学数据将有助于进一步研究，旨在了解农业领域中分子植物生理学的机制，并有助于实施减轻生物和非生物胁迫的有效策略。
