Department of Plant Protection Biology, Swedish University of Agricultural Sciences, PO Box 190, SE-234 22, Lomma, Sweden.
Department of Immunotechnology, Lund University, Sweden.
Plant Physiol Biochem. 2024 Oct;215:109032. doi: 10.1016/j.plaphy.2024.109032. Epub 2024 Aug 10.
There are limited molecular data and few biomarkers available for studies of field-grown plants, especially for plants grown during extremely long days. In this study we present quantitative proteomics data from 3 years of field trials on potato, conducted in northern and southern Sweden and analyze over 3000 proteins per year of the study and complement the proteomic analysis with metabolomic and transcriptomic analyses. Small but consistent differences linked to the longer days (an average of four more hours of light per day) in northern Sweden (20 h light/day) compared to southern Sweden can be observed, with a high correlation between the mRNA determined by RNA-seq and protein abundances. The majority of the proteins with differential abundances between northern and southern Sweden could be divided into three groups: metabolic enzymes (especially GABA metabolism), proteins involved in redox metabolism, and hydrolytic enzymes. The observed differences in metabolic enzyme abundances corresponded well with untargeted metabolite data determined by GC and LC mass-spectrometry. We also analyzed differences in protein abundance between potato varieties that performed relatively well in northern Sweden in terms of yield with those that performed relatively less well. This comparison indicates that the proteins with higher abundance in the high-yield quotient group are more anabolic in their character, whereas the proteins with lower abundance are more catabolic. Our results create a base of information about potato "field-omics" for improved understanding of physiological and molecular processes in field-grown plants, and our data indicate that the potato plant is not generally stressed by extremely long days.
关于野外生长植物的研究，尤其是在极长日照条件下生长的植物，可用的分子数据和生物标志物非常有限。在本研究中，我们提供了来自 3 年马铃薯田间试验的定量蛋白质组学数据，这些试验分别在瑞典北部和南部进行，并对研究过程中的 3000 多种蛋白质进行了分析，同时用代谢组学和转录组学分析对蛋白质组学分析进行了补充。与瑞典南部（每天 20 小时光照）相比，瑞典北部（每天平均多 4 小时光照）的长日照条件存在微小但一致的差异，通过 RNA-seq 确定的 mRNA 与蛋白质丰度之间具有高度相关性。在瑞典北部和南部之间丰度存在差异的大多数蛋白质可分为三组：代谢酶（特别是 GABA 代谢）、参与氧化还原代谢的蛋白质和水解酶。观察到的代谢酶丰度差异与通过 GC 和 LC 质谱法确定的非靶向代谢物数据非常吻合。我们还分析了在瑞典北部产量较高的马铃薯品种与产量较低的品种之间的蛋白质丰度差异。这一比较表明，在高产量系数组中丰度较高的蛋白质在性质上更具合成代谢特征，而丰度较低的蛋白质则更具分解代谢特征。我们的研究结果为马铃薯“田间组学”提供了信息基础，有助于更好地理解野外生长植物的生理和分子过程，并且我们的数据表明，马铃薯植株通常不会因极长的日照而受到压力。
