School of Public Health, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
Shanghai Key Laboratory of Compound Chinese Medicine, The Ministry of Education (MOE) Key Laboratory for Standardization of Chinese Medicine, Institute of Chinese Materia Medica, Shanghai University of Traditional Chinese Medicine, 1200 Cailun Road, Shanghai 201203, China.
Molecules. 2021 Oct 24;26(21):6422. doi: 10.3390/molecules26216422.
Cinnamon procyanidin oligomers (CPOs) are water-soluble components extracted from cinnamon. This study aims to explore the neuroprotection of B-type CPO (CPO-B) against 1-methyl-4-phenylpyridinium (MPP)-mediated cytotoxicity and the molecular mechanisms underlying its protection. The results demonstrated that CPO-B showed protection by increasing cell viability, attenuating an intracellular level of reactive oxygen species, downregulating cleaved caspase-3 expression, and upregulating the Bcl-2/Bax ratio. Moreover, CPO-B completely blocked the dephosphorylation of extracellular, signal-regulated kinase 1 and 2 (Erk1/2) caused by MPP. Treatment with an Erk1/2 inhibitor, SCH772984, significantly abolished the neuroprotection of CPO-B against MPP. Taken together, we demonstrate that CPO-B from cinnamon bark provided protection against MPP in cultured SH-SY5Y cells, and the potential mechanisms may be attributed to its ability to modulate the dysregulation between pro-apoptotic and anti-apoptotic proteins through the Erk1/2 signaling pathway. Our findings suggest that the addition of cinnamon to food or supplements might benefit patients with PD.
肉桂原花青素低聚物（CPO）是从肉桂中提取的水溶性成分。本研究旨在探讨 B 型 CPO（CPO-B）对 1-甲基-4-苯基吡啶（MPP）介导的细胞毒性的神经保护作用及其保护机制。结果表明，CPO-B 通过增加细胞活力、减轻细胞内活性氧水平、下调裂解 caspase-3 的表达和上调 Bcl-2/Bax 比值来发挥保护作用。此外，CPO-B 完全阻断了 MPP 引起的细胞外信号调节激酶 1 和 2（Erk1/2）的去磷酸化。用 Erk1/2 抑制剂 SCH772984 处理，可显著消除 CPO-B 对 MPP 的神经保护作用。综上所述，我们证明了来自肉桂皮的 CPO-B 可提供对培养的 SH-SY5Y 细胞中 MPP 的保护，其潜在机制可能归因于其通过 Erk1/2 信号通路调节促凋亡和抗凋亡蛋白之间的失调的能力。我们的研究结果表明，在食物或补充剂中添加肉桂可能有益于 PD 患者。
