Tan Xiaomiao, Zhu Jiangyu, Wakisaka Minato
Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Fukuoka, 808-0196, Japan.
School of Food Science and Engineering, Yangzhou University, No. 196 Huayang West Road, Hanjiang District, Yangzhou, 225127, Jiangsu, China.
World J Microbiol Biotechnol. 2021 Nov 13;37(12):217. doi: 10.1007/s11274-021-03185-1.
A feasible approach against the low yield of microalgae biomass involves the use of a stimulator for microalgal growth. In this research, vanillic acid present in the hydrolysate of agricultural waste, was applied to the cultivation of unicellular microalga Euglena gracilis. At the optimal dosage of 800 mg L vanillic acid, biomass yield at treatment increased 2.08-fold. Correspondingly, the content of chlorophyll a and carotenoids was 3.48 and 2.69 fold than of the control ground, respectively. Increased in cell aspect ratio demonstrated that the alga was more active after vanillic acid treatment. Furthermore, relative lipid and carbohydrate content were analyzed using Fourier transform infrared spectroscopy, the result showed that vanillic acid increased the lipid content in algal cells without sacrificing biomass, which would be a promising way for future biofuel production.
一种解决微藻生物量产量低的可行方法是使用微藻生长刺激剂。在本研究中，将农业废弃物水解产物中存在的香草酸应用于单细胞微藻纤细裸藻的培养。在香草酸最佳剂量为800 mg/L时，处理组的生物量产量提高了2.08倍。相应地，叶绿素a和类胡萝卜素的含量分别是对照组的3.48倍和2.69倍。细胞长宽比的增加表明香草酸处理后藻类更活跃。此外，使用傅里叶变换红外光谱分析了相对脂质和碳水化合物含量，结果表明香草酸在不牺牲生物量的情况下增加了藻类细胞中的脂质含量，这将是未来生物燃料生产的一种有前景的方法。
