木质素基本结构中的酚类化合物对淡水微藻眼虫的生物量产量和脂质积累的增强作用。

Enhancement of biomass yield and lipid accumulation of freshwater microalga Euglena gracilis by phenolic compounds from basic structures of lignin.

机构信息

Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Fukuoka 808-0196, Japan.

Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Fukuoka 808-0196, Japan; School of Food Science and Engineering, Yangzhou University, No.196 Huayang West Road, Hanjiang District, Yangzhou City, Jiangsu Province 225127, China.

出版信息

Bioresour Technol. 2021 Feb;321:124441. doi: 10.1016/j.biortech.2020.124441. Epub 2020 Nov 24.

DOI:10.1016/j.biortech.2020.124441

PMID:33268047

Abstract

Introducing biomass-derived additives into microalgae cultivation to increase its yield has been regarded as a more cost-effective and environment-friendly method compared with gene-editing and nutrients supplementation. In this research, feasibility of three major phenolic compounds from lignin's basic structures (guaiacyl-, hydroxyphenyl- and syringyl- types) for freshwater microalga Euglena gracilis cultivation was evaluated. The results indicated that trans-4-hydroxy-3-methoxycinnamic acid (HMA), 4-hydroxybenzaldehyde (HBA), and syringaldehyde (SRA) could all promote microalgae growth in a phytohormone-like role, and the highest promotion effect was achieved under HMA treatment. HMA at 0.5 g·L enhanced the cell biomass yield by 2.30 times, while HBA and SRA at the concentration of 0.1 g·L increased the yield by 1.30 and 1.21 times, respectively. In addition, increased carotenoids and lipid biosynthesis were also observed under the treatments of phenolic compounds, which would contribute to the microalgae biofuel production, since the growth and lipid accumulation of E. gracilis were simultaneously enhanced.

摘要

将生物质衍生添加剂引入微藻培养中以提高产量被认为是一种比基因编辑和营养物补充更具成本效益和环保的方法。在这项研究中，评估了木质素基本结构（愈创木基型、羟基苯基型和丁香基型）中的三种主要酚类化合物（丁香醛型、对羟基苯甲醛和丁香醛）对淡水微藻眼虫的培养可行性。结果表明，反式-4-羟基-3-甲氧基肉桂酸（HMA）、4-羟基苯甲醛（HBA）和丁香醛（SRA）都可以发挥植物激素样的作用促进微藻生长，其中 HMA 的促进效果最高。0.5 g·L 的 HMA 使细胞生物量产量提高了 2.30 倍，而 0.1 g·L 的 HBA 和 SRA 使产量分别提高了 1.30 倍和 1.21 倍。此外，在酚类化合物处理下还观察到类胡萝卜素和脂质生物合成增加，这有助于微藻生物燃料的生产，因为眼虫的生长和脂质积累同时得到了增强。