STOML2 的表达通过上调 PAI-1 促进多发性骨髓瘤细胞的增殖和糖酵解。
Expression of STOML2 promotes proliferation and glycolysis of multiple myeloma cells via upregulating PAI-1.
机构信息
Department of Hematology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi City, 832008, Xinjiang Uygur Autonomous Region, China.
Department of Intensive Care Unit, Luzhou People's Hospital, Luzhou City, 646000, Sichuan Province, China.
出版信息
J Orthop Surg Res. 2021 Nov 13;16(1):667. doi: 10.1186/s13018-021-02819-2.
BACKGROUND
This study aimed to investigate the effects of STOML2 and the relationship between STOML2 and PAI-1 in the development of multiple myeloma (MM).
METHODS
Cell proliferation was tested using CCK-8 assay and cell colony formation assay. Glucose consumption, lactate production and ATP level were measured using commercial kits. The mRNA and protein expression were assessed using quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting, respectively.
RESULTS
Both mRNA and protein expression of STOML2 were upregulated in MM patients compared to healthy volunteers. CCK-8 and colony formation assays demonstrated that STOML2 silencing inhibited cell proliferation in MM cells. Knockdown of STOML2 reduced glucose consumption, lactate production and ATP/ADP ratios. STOML2 silencing by shSTOML2 led to reduced PAI-1 expression. Overexpression of PAI-1 reversed the inhibitory effects of shSTOML2 on MM cell growth.
CONCLUSION
Results from this study demonstrated that STOML2 silencing inhibits cell proliferation and glycolysis through downregulation of PAI-1 expression, suggesting a new therapeutic target for MM.
背景
本研究旨在探讨 STOML2 的作用及其与 PAI-1 在多发性骨髓瘤（MM）发病机制中的关系。
方法
通过 CCK-8 法和集落形成实验检测细胞增殖；采用试剂盒检测葡萄糖消耗、乳酸生成和 ATP 水平；通过实时定量聚合酶链反应（qRT-PCR）和蛋白质印迹法分别检测 mRNA 和蛋白表达。
结果
与健康志愿者相比，MM 患者的 STOML2 mRNA 和蛋白表达均上调。CCK-8 和集落形成实验表明，沉默 STOML2 可抑制 MM 细胞的增殖。敲低 STOML2 可降低葡萄糖消耗、乳酸生成和 ATP/ADP 比值。shSTOML2 下调 STOML2 表达可降低 PAI-1 表达。过表达 PAI-1 可逆转 shSTOML2 对 MM 细胞生长的抑制作用。
结论
本研究结果表明，沉默 STOML2 通过下调 PAI-1 表达抑制细胞增殖和糖酵解，为 MM 提供了一个新的治疗靶点。
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