Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan.
Department of Pharmacy Practice, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad, Pakistan.
J Ethnopharmacol. 2022 Mar 1;285:114825. doi: 10.1016/j.jep.2021.114825. Epub 2021 Nov 11.
Ziziphus Oxyphylla belongs to family Ziziphus and has been used traditionally in hypertension. It is enriched with quercetin and kaempferol derivatives, catechin and cyclopeptide alkaloids.
The current research evaluates the antihypertensive potential of aqueous methanolic extract of Z. oxyphylla (AMEZO) in NG-nitro-L-arginine methyl ester (LNAME) induced hypertension in rats.
Phytochemical analysis of AMEZO was carried out using high performance liquid chromatography (HPLC) and electrospray ionization mass spectrometry (ESI-MS/MS). Antihypertensive activities of AMEZO (200 and 400 mg/kg) and Kaempferol were assessed in L-NAME (185 μmol/kg, intraperitoneal) injected hypertensive rats. In normotensive rats, blood pressure was assessed using Power Lab data system. Serum and tissue samples were preserved for estimation of nitric oxide (NO), Cyclic guanosine monophosphate (cGMP), interleukin-6 (IL-6), tumor necrosis factor (TNF- α) and oxidative stress markers respectively. mRNA levels of eNOS, ACE, COX-2 and NF-kB genes were assessed through qPCR.
The HPLC and ESI-MS/MS identified kaempferol, quercetin, catechin, ceanothic acid, zizybernalic acid and oxyphylline F. Chronic administration of AMEZO and kaempferol in L-NAME induced hypertensive rats significantly (p < 0.001) reduced systolic, diastolic and mean blood pressure. AMEZO and kaempferol caused meaningfully improved (p < 0.001) serum NO and cGMP levels. AMEZO administration also noticeably decrease the elevated IL-6 and TNF- α concentration in hypertensive animals. Administration of AMEZO and kaempferol also improved oxidative stress markers (MDA, CAT, SOD, GSH). The antihypertensive activity of AMEZO also resulted in upregulation of eNOS and downregulation of ACE.
These data depict that AMEZO and kaempferol showed antihypertensive activity in LNAME induced hypertensive rats possibly mediated through improvement in NO and cGMP levels, modulation of mRNA expression of eNOS, ACE, COX-2 and NF-kB and suppression of oxidative stress related inflammatory markers, proposing a defensive role in cardiovascular diseases.
酸枣属于酸枣属，传统上用于治疗高血压。它富含槲皮素和山奈酚衍生物、儿茶素和环肽生物碱。
本研究评估了酸枣水甲醇提取物（AMEZO）在 L-NAME 诱导的高血压大鼠中的降压潜力。
采用高效液相色谱（HPLC）和电喷雾电离质谱（ESI-MS/MS）对 AMEZO 进行植物化学分析。在 L-NAME（185 μmol/kg，腹腔内）注射的高血压大鼠中评估 AMEZO（200 和 400 mg/kg）和山奈酚的降压活性。在正常血压大鼠中，使用 Power Lab 数据系统评估血压。分别保存血清和组织样本，用于估计一氧化氮（NO）、环磷酸鸟苷（cGMP）、白细胞介素-6（IL-6）、肿瘤坏死因子（TNF-α）和氧化应激标志物。通过 qPCR 评估 eNOS、ACE、COX-2 和 NF-kB 基因的 mRNA 水平。
HPLC 和 ESI-MS/MS 鉴定出山奈酚、槲皮素、儿茶素、苍术酸、枣醇酸和氧叶定 F。AMEZO 和山奈酚在 L-NAME 诱导的高血压大鼠中的慢性给药显著（p<0.001）降低收缩压、舒张压和平均血压。AMEZO 和山奈酚可显著改善（p<0.001）血清 NO 和 cGMP 水平。AMEZO 给药还可明显降低高血压动物中升高的 IL-6 和 TNF-α浓度。AMEZO 给药还改善了氧化应激标志物（MDA、CAT、SOD、GSH）。AMEZO 的降压活性还导致 eNOS 的上调和 ACE 的下调。
这些数据表明，AMEZO 和山奈酚在 LNAME 诱导的高血压大鼠中表现出降压活性，可能通过改善 NO 和 cGMP 水平、调节 eNOS、ACE、COX-2 和 NF-kB 的 mRNA 表达以及抑制氧化应激相关炎症标志物来介导，在心血管疾病中发挥保护作用。
Zotero 插件