Rana Reemal, Mehmood Malik Hassan, Shaukat Bushra, Shahid Sidra, Malik Abdul, Murtaza Babar
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Government College University, Faisalabad 38000, Pakistan.
Department of Pharmacology, College of pharmacy, University of Sargodha, Sargodha 40100, Pakistan.
Life (Basel). 2022 Nov 10;12(11):1842. doi: 10.3390/life12111842.
Based on the vernacular reputation of Coriandrum sativum and Citrus limon to treat hypertension, this study was designed to explore the cardiovascular effects of C. sativum (CS) and C. limon (CL) on arsenic-induced hypertension and endothelial damage. Hypertension was induced by arsenic (100 ppm) in drinking water. The crude methanolic extracts of CS and CL were tested for in vivo and in vitro activities using Power Lab. High performance liquid chromatography analysis of CS and CL showed the presence of phenolic compounds. In anesthetized rats, CS (50 mg) and CL (10 mg) showed a marked decrease in blood pressure of 51% and 35%, respectively. Similarly, ascorbic acid (10 mg) also showed a decreased blood pressure (41%). The CS and CL caused complete relaxation (0.003−5 mg/mL) against phenylephrine (1µM) and high K+ (80 mM)-induced contraction. The CS and CL, independently and in combination, exhibited marked (p < 0.001) attenuation in the blood pressure of the arsenic-induced hypertensive rats when compared with the controls. The beneficial effects of the CS and CL were also observed on lipid peroxidation and eNOS. These data suggest that CS and CL possess significant antihypertensive activity, possibly mediated via endothelium protection, and anti-oxidant effects. Thus, this study provides a rationale for the medicinal use of CS and CL in hypertension and also against arsenic-induced cardiovascular complications.
基于芫荽和柠檬在民间治疗高血压的声誉，本研究旨在探讨芫荽（CS）和柠檬（CL）对砷诱导的高血压和内皮损伤的心血管作用。通过在饮用水中添加砷（100 ppm）诱导高血压。使用Power Lab对CS和CL的粗甲醇提取物进行体内和体外活性测试。CS和CL的高效液相色谱分析表明存在酚类化合物。在麻醉大鼠中，CS（50 mg）和CL（10 mg）的血压分别显著降低了51%和35%。同样，抗坏血酸（10 mg）也使血压降低（41%）。CS和CL对去氧肾上腺素（1µM）和高钾（80 mM）诱导的收缩产生完全舒张作用（0.003−5 mg/mL）。与对照组相比，CS和CL单独及联合使用时，砷诱导的高血压大鼠血压均显著降低（p < 0.001）。在脂质过氧化和内皮型一氧化氮合酶方面也观察到了CS和CL的有益作用。这些数据表明，CS和CL具有显著的降压活性，可能通过内皮保护和抗氧化作用介导。因此，本研究为CS和CL在高血压治疗以及对抗砷诱导的心血管并发症中的药用提供了理论依据。
