Department of Oral Medicine, Nanjing Stomatological Hospital, Medical School of Nanjing University, Nanjing 210008, China.
Affiliated Stomatological Hospital of Xuzhou Medical University, Xuzhou 221002, China.
J Proteomics. 2022 Jan 16;251:104422. doi: 10.1016/j.jprot.2021.104422. Epub 2021 Nov 12.
Extracellular vesicles (EVs) are involved in a wide range of pathological processes and recognized as potential and novel biomarkers for oral squamous cell carcinoma (OSCC). Here, we describe the plasma EV proteome of rats with 4-nitroquinoline-1-oxide (4NQO)-induced OSCC or moderate dysplasia (MD), which can progress to OSCC, by tandem mass tag (TMT)-labeled mass spectrometry. The proteomic profiles suggest the differential expression of various proteins in MD and OSCC, some well-recognized pathological changes (e.g., translation, ATP metabolism, and mesenchymal transition), and some novel pathological changes (e.g., podosome, focal adhesion, and S100 binding). We re-examined the presence of traditional exosomal markers and the reported novel pan-EV markers. In summary, these results suggest potential EV biomarkers and underlying pathological changes in early OSCC as well as the presence of oral-derived EVs in plasma and the need for pan-EV markers. SIGNIFICANCE: This research suggests potential EV biomarkers and underlying pathological changes in early OSCC as well as the presence of oral-derived EVs in plasma and the need for pan-EV markers.
细胞外囊泡 (EVs) 参与广泛的病理过程，被认为是口腔鳞状细胞癌 (OSCC) 的潜在新型生物标志物。在这里，我们通过串联质量标签 (TMT) 标记质谱法描述了 4-硝基喹啉 1-氧化物 (4NQO) 诱导的 OSCC 或可能发展为 OSCC 的中度发育不良 (MD) 大鼠的血浆 EV 蛋白质组。蛋白质组学图谱表明 MD 和 OSCC 中各种蛋白质的差异表达，一些公认的病理变化（例如，翻译、ATP 代谢和间质转化），以及一些新的病理变化（例如，足突、黏着斑和 S100 结合）。我们重新检查了传统外泌体标志物和报道的新型泛 EV 标志物的存在情况。总之，这些结果表明早期 OSCC 中存在潜在的 EV 生物标志物和潜在的病理变化，以及血浆中存在口腔来源的 EV 和对泛 EV 标志物的需求。
本研究表明早期 OSCC 中存在潜在的 EV 生物标志物和潜在的病理变化，以及血浆中存在口腔来源的 EV 和对泛 EV 标志物的需求。
