Department of Neuroscience, Psychology, Drug Research and Child Health - NEUROFARBA - Section, University of Florence, Florence, Italy.
Department of Otorhinolaryngology, Careggi University Hospital, Florence, Italy.
J Oral Pathol Med. 2022 Apr;51(4):350-357. doi: 10.1111/jop.13263. Epub 2021 Dec 1.
The identification of early diagnostic and prognostic biomarkers in oral squamous cell carcinoma (OSCC) is an unmet clinical need. We hypothesized that extracellular vesicles miR-210 expression (EV-miR-210) could be a potential biomarker for OSCC diagnosis and follow-up.
The expression of EV-miR-210 was measured in the plasma of OSCC patients (n = 30) and compared to that of controls (n = 14).
The median EV-miR-210 expression was significantly higher in OSCC patients compared to controls who had often, undetectable levels (p < 0.0001). We performed receiver operating characteristic (ROC) analysis for discriminating OSCC cases from controls. EV-miR-210 yielded an area under the curve (AUC) of 0.9513 with sensitivity 92.3% and specificity 86.6%. Kaplan-Meier curves indicated that high EV-miR-210 expression was associated with worse 3 years' survival (p < 0.05). Cox regression hazard model indicated that high EV-miR-210, G2, and G3 grading and pathological nodal status (pN)>1 were independent predictors of worse survival in OSCC patients.
These preliminary data suggest that EV-mir-210 may be a novel diagnostic and prognostic biomarker in OSCC.
在口腔鳞状细胞癌（OSCC）中，鉴定早期诊断和预后生物标志物是一个未满足的临床需求。我们假设细胞外囊泡 miR-210 表达（EV-miR-210）可能是 OSCC 诊断和随访的潜在生物标志物。
测量了 OSCC 患者（n=30）和对照组（n=14）血浆中 EV-miR-210 的表达。
与对照组相比，OSCC 患者的 EV-miR-210 表达中位数明显更高，对照组通常检测不到 EV-miR-210 表达（p<0.0001）。我们进行了区分 OSCC 病例和对照组的接收者操作特征（ROC）分析。EV-miR-210 的曲线下面积（AUC）为 0.9513，具有 92.3%的敏感性和 86.6%的特异性。Kaplan-Meier 曲线表明，高 EV-miR-210 表达与较差的 3 年生存率相关（p<0.05）。Cox 回归风险模型表明，高 EV-miR-210、G2 和 G3 分级以及病理性淋巴结状态（pN>1）是 OSCC 患者生存较差的独立预测因子。
这些初步数据表明，EV-mir-210 可能是 OSCC 的一种新型诊断和预后生物标志物。
