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从 COVID-19 康复供体中鉴定 SARS-CoV-2“记忆”NK 细胞用于过继细胞治疗。

Identifying SARS-CoV-2 'memory' NK cells from COVID-19 convalescent donors for adoptive cell therapy.

机构信息

Research Unit, Basque Center for Blood Transfusion and Human Tissues, Osakidetza, Galdakao, Spain.

Cell Therapy, Stem Cells and Tissues Group, Biocruces Bizkaia Health Research Institute, Barakaldo, Spain.

出版信息

Immunology. 2022 Feb;165(2):234-249. doi: 10.1111/imm.13432. Epub 2021 Dec 2.

DOI:10.1111/imm.13432
PMID:34775592
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8652867/
Abstract

COVID-19 disease is the manifestation of syndrome coronavirus 2 (SARS-CoV-2) infection, which is causing a worldwide pandemic. This disease can lead to multiple and different symptoms, being lymphopenia associated with severity one of the most persistent. Natural killer cells (NK cells) are part of the innate immune system, being fighting against virus-infected cells one of their key roles. In this study, we determined the phenotype of NK cells after COVID-19 and the main characteristic of SARS-CoV-2-specific-like NK population in the blood of convalescent donors. CD57+ NKG2C+ phenotype in SARS-CoV-2 convalescent donors indicates the presence of 'memory'/activated NK cells as it has been shown for cytomegalovirus infections. Although the existence of this population is donor dependent, its expression may be crucial for the specific response against SARS-CoV-2, so that, it gives us a tool for selecting the best donors to produce off-the-shelf living drug for cell therapy to treat COVID-19 patients under the RELEASE clinical trial (NCT04578210).

摘要

COVID-19 疾病是综合征冠状病毒 2（SARS-CoV-2）感染的表现，正在导致全球大流行。这种疾病可能导致多种不同的症状，与严重程度相关的淋巴细胞减少症是最持久的症状之一。自然杀伤细胞（NK 细胞）是先天免疫系统的一部分，对抗病毒感染细胞是其主要作用之一。在这项研究中，我们确定了 COVID-19 后 NK 细胞的表型以及恢复期供体血液中 SARS-CoV-2 特异性样 NK 群体的主要特征。SARS-CoV-2 恢复期供体中的 CD57+NKG2C+表型表明存在“记忆”/激活的 NK 细胞，就像巨细胞病毒感染一样。尽管这种群体的存在取决于供体，但它的表达可能对针对 SARS-CoV-2 的特异性反应至关重要，因此，它为我们提供了一个工具，用于根据 RELEASE 临床试验（NCT04578210）选择最佳供体来生产现成的活药物，用于治疗 COVID-19 患者的细胞治疗。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b287/8652867/8695155d9475/IMM-165-234-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b287/8652867/9024a2eb4418/IMM-165-234-g007.jpg
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